1.1.1.218: morphine 6-dehydrogenase

This is an abbreviated version, for detailed information about morphine 6-dehydrogenase, go to the full flat file.

Reaction

morphine
+
NAD(P)+
=
morphinone
+
NAD(P)H
+
H+

Synonyms

morphine 6-dehydrogenase, naloxone reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.218 morphine 6-dehydrogenase

Engineering

Engineering on EC 1.1.1.218 - morphine 6-dehydrogenase

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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C80S
-
change of cysteine to serine: significantly improved stability
D47N
-
residue modified by site-directed mutagenesis, resulting in substantial loss of activity
K76M
-
residue modified by site-directed mutagenesis, resulting in substantial loss of activity
C80S
-
change of cysteine to serine: significantly improved stability
-
D47N
-
residue modified by site-directed mutagenesis, resulting in substantial loss of activity
-
K76M
-
residue modified by site-directed mutagenesis, resulting in substantial loss of activity
-
Y52F
-
F is incapable of acting as proton donor /aim: NAD+-dependent morphine dehydrogenase for biotransformation morphine-morphinone-hydromorphine, codeine-hydrocodone respectively (medically useful semisynthetic opiates); residue modified by site-directed mutagenesis, resulting in substantial loss of activity
-