18.104.22.168: gluconate 2-dehydrogenase
This is an abbreviated version, for detailed information about gluconate 2-dehydrogenase, go to the full flat file.
2-keto-D-gluconate reductase, 2-keto-D-gluconate-yielding D-gluconate dehydrogenase, 2-ketoaldonate reductase, 2-ketogluconate reductase, 2KGA reductase, 2KGR, 2KR, FAD-GADH, GAD, GADH, gluconate dehydrogenase, Gox0417, reductase, 2-ketogluconate
Purification on EC 22.214.171.124 - gluconate 2-dehydrogenase
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affinity chromatography on Blue-Dextran Sepharose B
ammonium sulfate, DEAE-Toyopearl, Phenyl-Toyopearl, Blue-Sepharose, Sephacryl S-200
His6-tagged enzyme, metal-chelate affinity chromatography
native enzyme 67fold from membranes and bound to FAD by solubilization with 0.1% Triton X-100, anion chromatography, and three steps of hydrophobic interaction chromatography