1.1.1.215: gluconate 2-dehydrogenase

This is an abbreviated version, for detailed information about gluconate 2-dehydrogenase, go to the full flat file.

Reaction

D-gluconate
+
NADP+
=
2-dehydro-D-gluconate
+
NADPH
+
H+

Synonyms

2-keto-D-gluconate reductase, 2-keto-D-gluconate-yielding D-gluconate dehydrogenase, 2-ketoaldonate reductase, 2-ketogluconate reductase, 2KGA reductase, 2KGR, 2KR, FAD-GADH, GAD, GADH, gluconate dehydrogenase, Gox0417, reductase, 2-ketogluconate

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.215 gluconate 2-dehydrogenase

Purification

Purification on EC 1.1.1.215 - gluconate 2-dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity chromatography on Blue-Dextran Sepharose B
Acetobacter ascendens
-
ammonium sulfate, DEAE-Toyopearl, Phenyl-Toyopearl, Blue-Sepharose, Sephacryl S-200
Brevibacterium ketosoreductum
-
His6-tagged enzyme, metal-chelate affinity chromatography
-
native enzyme 67fold from membranes and bound to FAD by solubilization with 0.1% Triton X-100, anion chromatography, and three steps of hydrophobic interaction chromatography