1.1.1.215: gluconate 2-dehydrogenase

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For detailed information about gluconate 2-dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.215

1.1.1.215

2-keto-d-gluconic

gluconobacter

oxydans

5-keto-d-gluconate

gluconokinase

6-phosphogluconate

entner-doudoroff

l-idonate

cypripedii

2.7.1.12

synthesis

medicine

Reaction

Synonyms

2-HDH, 2-keto-D-gluconate reductase, 2-keto-D-gluconate-yielding D-gluconate dehydrogenase, 2-keto-L-gulonate reductase, 2-ketoaldonate reductase, 2-ketogluconate dehydrogenase, 2-ketogluconate reductase, 2KGA reductase, 2KGR, 2KR, FAD-GADH, GA2DH, GAD, GADH, gluconate dehydrogenase, gluconate-2-dehydrogenase, gluconate-2-DH, GluD, Gox0417, GOX1230, HDH, L-2-hydroxyacid dehydrogenase, NADP-gluconate 2-dehydrogenase, NADPH requiring 2-keto-L-gulonate reductase, reductase, 2-ketogluconate

ECTree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.1.215 gluconate 2-dehydrogenase

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Results	in table
1	Activating Compound
575	AA Sequence
6	Application
1	CAS Registry Number
12	Cloned(Commentary)
39	Cofactor
6	Crystallization (Commentary)
2	Expression
16	General Information
6	General Stability
19	Inhibitors
5	kcat/KM [mM/s]
27	KM Value [mM]
22	Localization
8	Metals/Ions
20	Molecular Weight [Da]
27	Natural Substrates/ Products (Substrates)
10	Organic Solvent Stability
44	Organism
6	Pathway
18	pH Optimum
5	pH Range
6	pH Stability
2	pI Value
11	Protein Variants
10	Purification (Commentary)
1	Reaction
3	Reaction Type
37	Reference
2	Source Tissue
8	Specific Activity [micromol/min/mg]
8	Storage Stability
104	Substrates and Products (Substrate)
15	Subunits
57	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
2	Temperature Range [°C]
9	Temperature Stability [°C]
3	Turnover Number [1/s]

Purification

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Purification on EC 1.1.1.215 - gluconate 2-dehydrogenase

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2 entries

affinity chromatography on Blue-Dextran Sepharose B

Acetobacter ascendens

286274, 286275

ammonium sulfate, DEAE-Toyopearl, Phenyl-Toyopearl, Blue-Sepharose, Sephacryl S-200

Brevibacterium ketosoreductum

286278

His6-tagged enzyme, metal-chelate affinity chromatography

Escherichia coli

286279

native enzyme 67fold from membranes and bound to FAD by solubilization with 0.1% Triton X-100, anion chromatography, and three steps of hydrophobic interaction chromatography

Gluconobacter frateurii

A4PIB0

684567

native enzyme, via ultrasonification and ultracentrifugation, phase separation, ammonium sulfate precipitation, and DEAE-Sepharose and hydroxyapatite chromatography

Pseudomonas plecoglossicida

A0A2H4PJM1; A0A2H4PJM2; A0A2H4PJM7

760370

partial

Gluconobacter oxydans

286275

recombinant His6-tagged enzyme from Escherichia coli by nickel affinity and anion exchange chromatography, followed by gel filtration

Ketogulonicigenium vulgare

742168