18.104.22.168: gluconate 2-dehydrogenase
This is an abbreviated version, for detailed information about gluconate 2-dehydrogenase, go to the full flat file.
2-HDH, 2-keto-D-gluconate reductase, 2-keto-D-gluconate-yielding D-gluconate dehydrogenase, 2-ketoaldonate reductase, 2-ketogluconate dehydrogenase, 2-ketogluconate reductase, 2KGA reductase, 2KGR, 2KR, FAD-GADH, GA2DH, GAD, GADH, gluconate dehydrogenase, gluconate-2-dehydrogenase, GluD, Gox0417, GOX1230, HDH, L-2-hydroxyacid dehydrogenase, reductase, 2-ketogluconate
Purification on EC 22.214.171.124 - gluconate 2-dehydrogenase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
affinity chromatography on Blue-Dextran Sepharose B
ammonium sulfate, DEAE-Toyopearl, Phenyl-Toyopearl, Blue-Sepharose, Sephacryl S-200
His6-tagged enzyme, metal-chelate affinity chromatography
native enzyme 67fold from membranes and bound to FAD by solubilization with 0.1% Triton X-100, anion chromatography, and three steps of hydrophobic interaction chromatography
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity and anion exchange chromatography, followed by gel filtration