1.1.1.215: gluconate 2-dehydrogenase

This is an abbreviated version, for detailed information about gluconate 2-dehydrogenase, go to the full flat file.

Reaction

D-gluconate
+
NADP+
=
2-dehydro-D-gluconate
+
NADPH
+
H+

Synonyms

2-HDH, 2-keto-D-gluconate reductase, 2-keto-D-gluconate-yielding D-gluconate dehydrogenase, 2-ketoaldonate reductase, 2-ketogluconate dehydrogenase, 2-ketogluconate reductase, 2KGA reductase, 2KGR, 2KR, FAD-GADH, GA2DH, GAD, GADH, gluconate dehydrogenase, gluconate-2-dehydrogenase, GluD, Gox0417, GOX1230, HDH, L-2-hydroxyacid dehydrogenase, reductase, 2-ketogluconate

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.215 gluconate 2-dehydrogenase

Crystallization

Crystallization on EC 1.1.1.215 - gluconate 2-dehydrogenase

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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant enzyme, sitting drop vapour diffusion method, mixing of 0.001 ml of 30 mg/ml protein in 25 mM Tris/HCl, pH 8.0, 0.3 M NaCl, and 1 mM dithiothreitol, with 0.001 ml of reservoir solution containing 23% w/v PEG 3350, 0.2 M MgCl2, and 0.1 M HEPES, pH 7.0, and equilibration against 0.1 ml of reservoir solution, 20°C, X-ray diffraction structure determination and analysis at 1.64 A resolution, molecular replacement and modeling
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