1.1.1.215: gluconate 2-dehydrogenase

This is an abbreviated version, for detailed information about gluconate 2-dehydrogenase, go to the full flat file.

Reaction

D-gluconate
+
NADP+
=
2-dehydro-D-gluconate
+
NADPH
+
H+

Synonyms

2-keto-D-gluconate reductase, 2-keto-D-gluconate-yielding D-gluconate dehydrogenase, 2-ketoaldonate reductase, 2-ketogluconate dehydrogenase, 2-ketogluconate reductase, 2KGA reductase, 2KGR, 2KR, FAD-GADH, GA2DH, GAD, GADH, gluconate dehydrogenase, gluconate-2-dehydrogenase, GluD, Gox0417, GOX1230, reductase, 2-ketogluconate

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.215 gluconate 2-dehydrogenase

Cloned

Cloned on EC 1.1.1.215 - gluconate 2-dehydrogenase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant large subunits
DNA and amino acid sequence determination and analysis, overexpression of wild-type and expression of start codon mutated enzyme in Escherichia coli strain DH5alpha
expressed in Escherichia coli
-
expression in Escherichia coli
expression in Saccharomyces cerevisiae
gene ga2dh, recombinant overexpression of the enzyme in Gluconobacter oxydans strain DSM2003 under the control of three promoters, PtufB, Pga2dh or Pghp0169, respectively. Recombinant strain Gluconobacter oxydans_tufB_ga2dh shows a similar growth rate to that of the control strain and displays the highest specific productivity of 2-dehydro-D-gluconate from D-gluconate, which is increased nearly twofold compared with that of the control strain during batch biotransformation, method optimization
mutagenized cj0414 and cj0415 alleles are introduced into Campylobacter jejuni 81-176
-