1.1.1.209: 3(or 17)alpha-hydroxysteroid dehydrogenase

This is an abbreviated version, for detailed information about 3(or 17)alpha-hydroxysteroid dehydrogenase, go to the full flat file.

Reaction

androsterone
+
NAD(P)+
=
5alpha-androstane-3,17-dione
+
NAD(P)H
+
H+

Synonyms

3(17)alpha-hydroxysteroid dehydrogenase, AKR1C21, dehydrogenase, 3(17)alpha-hydroxy steroid, More

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.209 3(or 17)alpha-hydroxysteroid dehydrogenase

Engineering

Engineering on EC 1.1.1.209 - 3(or 17)alpha-hydroxysteroid dehydrogenase

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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G225P/G226P
-
reduced affinity (Km) for both 3alpha- and 17alpha-hydroxysteroid substrates by up to 160fold, shows high selectivity for 5beta-pregnane-3alpha,20alpha-diol in the oxidation of the 3alpha-hydroxy group and, conversely, broad specificity for both 17alpha- and 17beta-hydroxysteroids
Q222N
-
mutation of residue involved in forming the safety belt for binding of the coenzyme. Kinetic constants for 3-hydroxy/3-ketosteroids and 17-hydroxy/ketosteroids similar to wild type
Y224D
-
mutation reduces the Km value for NADP(H) by up to 80fold and completely reverses the 17alpha stereospecificity of the enzyme
Y224F
-
mutation of residue involved in forming the safety belt for binding of the coenzyme. Kinetic constants for 3-hydroxy/3-ketosteroids and 17-hydroxy/ketosteroids similar to wild type