1.1.1.206: tropinone reductase I

This is an abbreviated version, for detailed information about tropinone reductase I, go to the full flat file.

Reaction

Tropine
+
NADP+
=
tropinone
+
NADPH
+
H+

Synonyms

BaTRI, dehydrogenase, tropine, DnTR1, DsTRI, More, TR-I, TR1, TRI, tropine biosynthetic tropinone reductase, Tropine dehydrogenase, tropine-forming reductase, tropine-forming tropinone reductase, tropinone reductase, tropinone reductase I, tropinone reductase II, tropinone reductase-I, WcTR-I, WsTR-I, WsTR-I protein

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.206 tropinone reductase I

Temperature Stability

Temperature Stability on EC 1.1.1.206 - tropinone reductase I

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
loss of 65% activity after 60 hours in crude extracts; loss of 90% activity of the purified enzyme after 10 hours, presence of 10% ethanol during the purification procedure
50 - 80
purified recombinant His-tagged enzyme, pH 6.6, 30 min, over 92% activity remaining at up to 50C, 30% remaining at 60C, 7% at 70C, complete inactivation occurs at 80C
50
purified recombinant enzyme, 30 min, over 92% of maximal activity remaining
50 - 60
purified recombinant enzyme, retains about 75% of maximal catalytic activity at up to 50C, 4.27% activity remaining on incubation at 60C
75
-
immediate thermal denaturation
80
purified recombinant enzyme, 30 min, inactivation