1.1.1.206: tropinone reductase I

This is an abbreviated version, for detailed information about tropinone reductase I, go to the full flat file.

Reaction

Tropine
+
NADP+
=
tropinone
+
NADPH
+
H+

Synonyms

dehydrogenase, tropine, More, TR-I, TRI, Tropine dehydrogenase, tropine-forming tropinone reductase, tropinone reductase, tropinone reductase I, tropinone reductase II

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.206 tropinone reductase I

Cloned

Cloned on EC 1.1.1.206 - tropinone reductase I

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cDNAs encoding two enzymes of tropane-alkaloid biosynthesis, tropinone reductase I and hyoscyamine-6beta-hydroxylase are simultaneously introduced into Nicotiana tabacum using particle bombardment and expressed under the control of the CaMV 35S promoter. The expression leads to the formation of unexpected substances, e.g. acetyltropine. Leaves of transgenic plants show in most cases higher nicotine content than leaves of control plants. In addition nicotine related compounds such as anatabine, nornicotine, bipyridine, anabasine, and myosmine are identified in transgenic tobacco lines and are below detection limit in wild-type plants
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gene tr, DNA and amino acid sequence determination and analysis, sequence comparison
gene TR1, expression in Atropa belladonna root cultures, derived from transfected leaves using the transformation system via Agrobacterium rhizogenes strain 15834
gene trI, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3)
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gene TRI, isolated from aerial tissue, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overexpression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3)
sixteen different peptide segment exchanges with tropinone reductase II
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TRI-transformed hairy root lines. Full-length fragment of TRI cDNA insert constructed into the pBI121 expression cassette in place of GUS gene. The vector pCAMBIA1304+ contains the TRI gene under control of CaMV35S promoter. Disarmed Agrobacterium tumefaciens strain C58C1 harboring both Agrobacterium rhizogenes Ri plasmid pRiA4 and pCAMBIA1304+, containing a single TRI gene, used for plant transformation
wild-type and recombinant enzymes from Atropa belladonna root cultures by ammonium sulfate fractionation
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