1.1.1.205: IMP dehydrogenase

This is an abbreviated version, for detailed information about IMP dehydrogenase, go to the full flat file.

Reaction

IMP
+
NAD+
+
H2O
=
XMP
+
NADH
+
H+

Synonyms

dehydrogenase, inosinate, EC 1.2.1.14, guaB2, IMD2, IMD3, IMD4, IMP dehydrogenase, IMP DH, IMP oxidoreductase, IMP-DH, IMP:NAD oxidoreductase, IMP:NAD+ oxidoreductase, IMPD, IMPDH, IMPDH II, IMPDH-1, IMPDH-B, IMPDH-S, IMPDH1, IMPDH2, inosinate dehydrogenase, inosine 5' monophosphate dehydrogenase, inosine 5'-monophosphate dehydrogenase, inosine 5'-monophosphate dehydrogenase 2, inosine 5'-phosphate dehydrogenase, inosine 5-monophosphate dehydrogenase, inosine 5-monophosphate dehydrogenase type I, inosine 5’ -monophosphate dehydrogenase, inosine monophosphate dehydrogenase, inosine monophosphate oxidoreductase, inosine-5'-monophosphate dehydrogenase, inosine-5'-phosphate dehydrogenase, inosinic acid 5’-monophosphate dehydrogenase, inosinic acid dehydrogenase, mpaF, Raspberry protein, SOI12, Superoxide-inducible protein 12, type 1 inosine monophosphate, type 2 inosine monophosphate dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.205 IMP dehydrogenase

Storage Stability

Storage Stability on EC 1.1.1.205 - IMP dehydrogenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, 10% loss of activity within 2 weeks and 40% loss after 6 weeks
-
-17°C no loss of activity for several months
-
-17°C, 24 h, 10% loss of activity
-
-20, no significant loss of activity for serveral weeks after ammonium sulfate precipitation, no loss of activity after Sephacryl S400 chromatography
-
-20°C
-20°C , no appreciable loss of activity for a few weeks in 20-25% glycerol
-
-70°C, 10 mg/ml bovine serum albumin, 3 weeks, 15% loss of activity
-
-80°C, can be indefinitely stored without loss of activity
-
-80°C, most activity is lost
-
-80°C, purified protein remains active for more than 18 months
-
0°C, p-chloromercuribenzoate, several weeks without loss of activity
-
2°C, 50% loss of activity after 2-3 days in dilutions with water
-
4°C, 24 h, 13% loss of activity
-
4°C, NAD+, IMP, 20% loss of activity after 18 h
-
4°C, no loss of activity after 3 days
-
frozen in 5 mM mercaptoethanol, 3 months, 50% loss of activity
-
frozen, 2 weeks, no loss of activity
-