1.1.1.205: IMP dehydrogenase

This is an abbreviated version, for detailed information about IMP dehydrogenase, go to the full flat file.

Reaction

IMP
+
NAD+
+
H2O
=
XMP
+
NADH
+
H+

Synonyms

dehydrogenase, inosinate, EC 1.2.1.14, guaB2, IMD2, IMD3, IMD4, IMP dehydrogenase, IMP DH, IMP oxidoreductase, IMP-DH, IMP:NAD oxidoreductase, IMP:NAD+ oxidoreductase, IMPD, IMPDH, IMPDH II, IMPDH-1, IMPDH-B, IMPDH-S, IMPDH1, IMPDH2, inosinate dehydrogenase, inosine 5' monophosphate dehydrogenase, inosine 5'-monophosphate dehydrogenase, inosine 5'-monophosphate dehydrogenase 2, inosine 5'-phosphate dehydrogenase, inosine 5-monophosphate dehydrogenase, inosine 5-monophosphate dehydrogenase type I, inosine 5’ -monophosphate dehydrogenase, inosine monophosphate dehydrogenase, inosine monophosphate oxidoreductase, inosine-5'-monophosphate dehydrogenase, inosine-5'-phosphate dehydrogenase, inosinic acid 5’-monophosphate dehydrogenase, inosinic acid dehydrogenase, mpaF, Raspberry protein, SOI12, Superoxide-inducible protein 12, type 1 inosine monophosphate, type 2 inosine monophosphate dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.205 IMP dehydrogenase

Purification

Purification on EC 1.1.1.205 - IMP dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
95% homogeneity
-
; native enzyme over 140fold from nitrogen-fixing nodules by ammonium sulfate fractionation, gel filtration, and affinity chromatography to homogeneity
-
affinity chromatography; homogeneity; native enzyme about 20fold to homogeneity by ammonium sulfate fractionation, gel filtration, and affinity chromatography
-
affinity chromatography; large scale
-
affinity chromatography; native enzyme about 14fold by affinity chromatography on different immobilized dyes, elution by salt gradient, over 90% purity of the enzyme, method development and optimization, overview
-
by Ni-NTA affinity chromatography
-
derepressed mutant
-
hepatoma cells 3924 A
-
hepatoma cells 3924 A; homogeneitiy
-
homogeneitiy
-
homogeneity
MOLT 4F human T-lymphoblasts
-
native enzyme from hepatoma 3924A cells to homogeneity
-
native enzyme from MCF7 cells partially by gel filtration, recombinant His-tagged type II IMPDH from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
P-388 lymphocytic leucemia tumor cells
-
partial purification
partially 20fold by ammonium sulfate fractionation; partial purification
-
partially by ammonium sulfate fractionation; partial purification
-
recombinant enzyme from wild-type and mutant enzyme-deficient Escherichia coli strain H712
recombinant FLAG-tagged large splicing variant by immunoaffinity
recombinant full-length enzyme and alphabeta core domain of the enzyme from Escherichia coli
-
recombinant full-length type I isozyme from Escherichia coli by two steps of affinity chromatography, recombinant His-tagged type I isozyme subdomain from Escherichia coli by metal affinity chromatography and ammonium sulfate fractionation
-
recombinant isoyzmes IMPDH I and IMPDH II from Escherichia coli strain BL21(DE3) by two steps of ion exchange chromatography
-
recombinant protein
recombinant wild-type and mutant IMPDH1s from Escherichia coli strain H712 by two steps of affinity chromatography, and gel filtration, to over 95% purity
recombnant enzyme
-
to more than 95% purity
-