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1.1.1.205: IMP dehydrogenase

This is an abbreviated version!
For detailed information about IMP dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.205

Reaction

IMP
+
NAD+
+
H2O
=
XMP
+
NADH
+
H+

Synonyms

A1S_3321, BTH_I2056, class I IMPDH, class II IMPDH, dehydrogenase, inosinate, DR63_268, EC 1.2.1.14, GBAA_0008, guaB, guaB2, IMD2, IMD3, IMD4, IMP dehydrogenase, IMP DH, IMP oxidoreductase, IMP-DH, IMP:NAD oxidoreductase, IMP:NAD+ oxidoreductase, IMPD, IMPDH, IMPDH II, IMPDH-1, IMPDH-B, IMPDH-S, IMPDH1, IMPDH2, IMPDHA, IMPDHab, IMPDHba, IMPDHbt, IMPDHI, IMPDHII, IMPDHkp, IMPDHlpp, IMPDHnm, IMPDHpa, inosinate dehydrogenase, inosine 5' monophosphate dehydrogenase, inosine 5'-monophosphate dehydrogenase, inosine 5'-monophosphate dehydrogenase 2, inosine 5'-phosphate dehydrogenase, inosine 5-monophosphate dehydrogenase, inosine 5-monophosphate dehydrogenase 2, inosine 5-monophosphate dehydrogenase type I, inosine 5’ -monophosphate dehydrogenase, inosine 5’-monophosphate dehydrogenase 2, inosine monophosphate dehydrogenase, inosine monophosphate dehydrogenase-1, inosine monophosphate dehydrogenase-2, inosine monophosphate oxidoreductase, inosine-5'-monophosphate dehydrogenase, inosine-5'-phosphate dehydrogenase, inosine-5-monophosphate dehydrogenase, inosinic acid 5’-monophosphate dehydrogenase, inosinic acid dehydrogenase, lpg1723, mpaF, NMC1103, Raspberry protein, Rv3411c, SOI12, Superoxide-inducible protein 12, type 1 inosine monophosphate, type 2 inosine monophosphate dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.205 IMP dehydrogenase

Purification

Purification on EC 1.1.1.205 - IMP dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
95% homogeneity
-
affinity chromatography
-
by Ni-NTA affinity chromatography
-
derepressed mutant
-
hepatoma cells 3924 A
-
HisTrap column chromatography and Sephacryl S-300 gel filtration
HisTrap column chromatography and Superose 6 gel filtration
homogeneity
large scale
-
MOLT 4F human T-lymphoblasts
-
native enzyme about 14fold by affinity chromatography on different immobilized dyes, elution by salt gradient, over 90% purity of the enzyme, method development and optimization, overview
-
native enzyme about 20fold to homogeneity by ammonium sulfate fractionation, gel filtration, and affinity chromatography
-
native enzyme from hepatoma 3924A cells to homogeneity
-
native enzyme from MCF7 cells partially by gel filtration, recombinant His-tagged type II IMPDH from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
native enzyme over 140fold from nitrogen-fixing nodules by ammonium sulfate fractionation, gel filtration, and affinity chromatography to homogeneity
-
Ni-NTA agarose resin column chromatography
Ni-NTA bead chromatography
-
Ni-NTA Sepharose bead chromatography and Superdex 200 gel filtration
P-388 lymphocytic leucemia tumor cells
-
partial purification
partially 20fold by ammonium sulfate fractionation
-
partially by ammonium sulfate fractionation
-
recombinant enzyme from wild-type and mutant enzyme-deficient Escherichia coli strain H712
recombinant FLAG-tagged large splicing variant by immunoaffinity
recombinant full-length enzyme and alphabeta core domain of the enzyme from Escherichia coli
-
recombinant full-length type I isozyme from Escherichia coli by two steps of affinity chromatography, recombinant His-tagged type I isozyme subdomain from Escherichia coli by metal affinity chromatography and ammonium sulfate fractionation
-
recombinant His-tagged long and short enzyme CBS-deletion mutants, BaIMPDHDELTAL and BaIMPDHDELTAS, from Escherichia coli by nickel affinity chromatography , tag cleavage by TEV protease, and removal by another step of nickel affinity chromatography, followed by dialysis
-
recombinant His-tagged long enzyme CBS-deletion mutant, ClpIMPDHDELTAL, from Escherichia coli by nickel affinity chromatography , tag cleavage by TEV protease, and removal by another step of nickel affinity chromatography, followed by dialysis
-
recombinant His-tagged long enzyme CBS-deletion mutant, VcIMPDHDELTAL, from Escherichia coli by nickel affinity chromatography , tag cleavage by TEV protease, and removal by another step of nickel affinity chromatography, followed by dialysis
-
recombinant His-tagged short enzyme CBS-deletion mutant, CjIMPDHDELTAS, from Escherichia coli by nickel affinity chromatography , tag cleavage by TEV protease, and removal by another step of nickel affinity chromatography, followed by dialysis
-
recombinant isoyzmes IMPDH I and IMPDH II from Escherichia coli strain BL21(DE3) by two steps of ion exchange chromatography
-
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, His6-tag cleavage using recombinant TEV protease, and additional nickel affinity chromatography to remove the protease, the uncut protein and the affinity tag, followed by dialysis
recombinant protein
recombinant soluble enzyme 1.7fold from Escherichia coli strain C41(DE3) by affinity chromatography and gel filtration
recombinant soluble His-tagged enzyme from Escherichia coli strain BL21(DE3)/pDIA17 by nickel affinity chromatography and gel filtration
recombinant wild-type and mutant enzymes from Escherichia coli by affinity chromatography and gel filtration
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)
recombinant wild-type and mutant IMPDH1s from Escherichia coli strain H712 by two steps of affinity chromatography, and gel filtration, to over 95% purity
to more than 95% purity
-