1.1.1.205: IMP dehydrogenase

This is an abbreviated version, for detailed information about IMP dehydrogenase, go to the full flat file.

Reaction

IMP
+
NAD+
+
H2O
=
XMP
+
NADH
+
H+

Synonyms

dehydrogenase, inosinate, EC 1.2.1.14, guaB2, IMD2, IMD3, IMD4, IMP dehydrogenase, IMP DH, IMP oxidoreductase, IMP-DH, IMP:NAD oxidoreductase, IMP:NAD+ oxidoreductase, IMPD, IMPDH, IMPDH II, IMPDH-1, IMPDH-B, IMPDH-S, IMPDH1, IMPDH2, inosinate dehydrogenase, inosine 5' monophosphate dehydrogenase, inosine 5'-monophosphate dehydrogenase, inosine 5'-monophosphate dehydrogenase 2, inosine 5'-phosphate dehydrogenase, inosine 5-monophosphate dehydrogenase, inosine 5-monophosphate dehydrogenase type I, inosine 5’ -monophosphate dehydrogenase, inosine monophosphate dehydrogenase, inosine monophosphate oxidoreductase, inosine-5'-monophosphate dehydrogenase, inosine-5'-phosphate dehydrogenase, inosinic acid 5’-monophosphate dehydrogenase, inosinic acid dehydrogenase, mpaF, Raspberry protein, SOI12, Superoxide-inducible protein 12, type 1 inosine monophosphate, type 2 inosine monophosphate dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.205 IMP dehydrogenase

Molecular Weight

Molecular Weight on EC 1.1.1.205 - IMP dehydrogenase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
-
2 * 38000, amino acid analysis
40000
-
x * 62000 + x * 40000, gel filtration in presence of guanidine
43640
-
calculated from amino acid composition
52200
-
(His)6 IMPDH protein, Western blot analysis
52330
-
MALDI-MS
52700
-
SDS-PAGE
52900
x * 52900, calculated from sequence
53770
-
calculated from amino acid composition
54000
-
SDS-PAGE
55260
-
type I, calculated from amino acid sequence
55390
-
calculated from amino acid sequence
55560
-
calculated from amino acid sequence
55680
-
type II, calculated from amino acid sequence
56280
-
calculated from amino acid sequence
62000
-
x * 62000 + x * 40000, gel filtration in presence of guanidine
65000
-
x * 56000, canonical isoforms of IMPDH1 and smaller retinal isoform, x * 65000, large retinal isoform, calculated
68000
-
2 * 68000, Yoshida sarcoma ascites tumor cells, SDS-PAGE
86000
-
smallest active species has a molecular weight of 86000, after polymerization in the absence of reducing agents the largest species has a molecular weight of 248000, gel filtration
90000 - 100000
-
basic catalytic molecular species, the species of this MW are of more than one type, under following conditions 2 species of indicated MW are present in approximately equal amount: 56000 and 95000 in 0.02 M phosphate at pH 7.4, 185000 and 300000 in 0.1 M Tris-citrate at pH 8.1, 185000 and 300000 in 0.02 M Tris-citrate at pH 7.4 or 8.1 containing 0.1 M KCl, 0.1 M NaCl or 0.25 M KCl, 90000 and 180000 in 0.38 M Tris-HCl, ultracentrifugation, sucrose density gradient sedimentation
127000
-
Yoshida sarcoma ascites tumor cells, gel filtration in presence of 10% (NH4)2SO4
165000
-
sedimentation equilibrium
200000
204000
-
gel filtration
225000
-
gel filtration
232000
-
gel filtration
245000
-
rat hepatoma 3924 A cells, gel filtration
249000
-
gel filtration, smallest enzymatically active species
389000
sedimentation velocity analysis
420000
gel fitlration
additional information