1.1.1.203: uronate dehydrogenase

This is an abbreviated version, for detailed information about uronate dehydrogenase, go to the full flat file.

Reaction

beta-D-galacturonate
+
NAD+
=
D-galactaro-1,5-lactone
+
NADH
+
H+

Synonyms

Atu3143, CsUDH, PfUDH, PmUDH, PnUDH, PSPTO_1053, PsUDH, Udh, uronate dehydrogenase, uronate:NAD-oxidoreductase, uronic acid dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.203 uronate dehydrogenase

Cloned

Cloned on EC 1.1.1.203 - uronate dehydrogenase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli; Udh subcloned into vector pTrc99A to produce pT1053 and transformed into Escherichia coli BL21 Star (DE3) to construct an Escherichia coli strain carrying INO1, MIOX, and udh
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene udh, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
udh gene from genomic DNA amplified and inserted into pET21b containing a six-His tag and expressed in Escherichia coli BL21(DE3) or inserted into vector pTrc99SE and transformed into Escherichia coli mutant strain MG1655 DELTAuxaC