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1.1.1.203: uronate dehydrogenase

This is an abbreviated version!
For detailed information about uronate dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.203

Reaction

beta-D-galacturonate
+
NAD+
=
D-galactaro-1,5-lactone
+
NADH
+
H+

Synonyms

Atu3143, CsUDH, D-galacturonate (uronate) dehydrogenase, D-GalUA oxidoreductase, EC 1.2.1.35, galacturonate acid reductase, galacturonate oxidoreductase, GalUR, Gor, PfUDH, PmUDH, PnUDH, poly-d-galacturonase, PSPTO_1053, PsUDH, Udh, uronate dehydrogenase, uronate:NAD-oxidoreductase, uronic acid dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.203 uronate dehydrogenase

Cloned

Cloned on EC 1.1.1.203 - uronate dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
co-expressed with myo-inositol oxygenase 4 from Arabidopsis thaliana in Saccharomyces cerevisiaen strain BY4741
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli DH5alpha cells
-
expressed in Escherichia coli Rosetta 2 (DE3) cells
-
expression in Escherichia coli
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene udh, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
udh gene from genomic DNA amplified and inserted into pET21b containing a six-His tag and expressed in Escherichia coli BL21(DE3) or inserted into vector pTrc99SE and transformed into Escherichia coli mutant strain MG1655 DELTAuxaC
Udh subcloned into vector pTrc99A to produce pT1053 and transformed into Escherichia coli BL21 Star (DE3) to construct an Escherichia coli strain carrying INO1, MIOX, and udh