1.1.1.201: 7beta-hydroxysteroid dehydrogenase (NADP+)
This is an abbreviated version!
For detailed information about 7beta-hydroxysteroid dehydrogenase (NADP+), go to the full flat file.
Word Map on EC 1.1.1.201
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1.1.1.201
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synthesis
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ursodeoxycholic
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taurinates
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dehydrogenases
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nadph-dependent
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aerofaciens
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bile
- 1.1.1.201
- synthesis
-
ursodeoxycholic
-
taurinates
- dehydrogenases
-
nadph-dependent
- aerofaciens
- bile
Reaction
Synonyms
7beta-HSD, 7beta-HSDH, 7beta-hydroxysteroid dehydrogenase, More, NADP-dependent 7beta-hydroxysteroid dehydrogenase, RUMGNA_02585
ECTree
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Crystallization
Crystallization on EC 1.1.1.201 - 7beta-hydroxysteroid dehydrogenase (NADP+)
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enzyme bound to NADP+, sitting drop vapor diffusion method, using 0.1 M sodium malonate pH 7.0, 12% (w/v) polyethylene glycol 3350
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purified His-tagged enzyme complexed with NADP+, mixing of 10-15 mg/ml protein in 20% v/v glycerol, 20 mM potassium phosphate, pH 7.0, and 5 mM NADP+, with1 M sodium formate, 100 mM sodium acetate, pH 4.6, at room temperature, 3-4days, X-ray diffraction structure determination and analysis at 1.6 A resolution, modelling, molecular replacement using the the apoform structure of 7alpha-HSDH from Escherichia coli as search model, PDB ID 1AHH