1.1.1.198: (+)-borneol dehydrogenase

This is an abbreviated version, for detailed information about (+)-borneol dehydrogenase, go to the full flat file.

Reaction

(+)-borneol
+
NAD+
=
(+)-Camphor
+
NADH
+
H+

Synonyms

AaBDH, ADH2, BDH, bicyclic monoterpenol dehydrogenase, THL1_4180

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.198 (+)-borneol dehydrogenase

Purification

Purification on EC 1.1.1.198 - (+)-borneol dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
near homogeneity, chromatography techniques, affinity chromatography
-
recombinant enzyme refolded from inclusion bodies after expression in Escherichia coli strain BL21 by anion exchange chromatography and dialysis for refolding, native enzyme from strain TCU-HL1 by hydrophobic interaction and anion exchange chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration