1.1.1.195: cinnamyl-alcohol dehydrogenase

This is an abbreviated version, for detailed information about cinnamyl-alcohol dehydrogenase, go to the full flat file.

Reaction

cinnamyl alcohol
+
NADP+
=
cinnamaldehyde
+
NADPH
+
H+

Synonyms

ADH, AdhA, alcohol dehydrogenase, Bmr6, brown midrib6, Brown-midrib 1 protein, CAD, CAD 7/8, CAD1, CAD10, CAD11, CAD12, CAD13, CAD14, CAD15, CAD2, CAD3, CAD4, CAD5, CAD6, CAD7, CAD8, CAD9, CADH I, cinnamyl alcohol dehydrogenase, cinnamyl alcohol dehydrogenase 1, cinnamyl alcohol dehydrogenase 2, dehydrogenase, cinnamyl alcohol, FC1, FLEXIBLE CULM1, More, ScAdh6p

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.195 cinnamyl-alcohol dehydrogenase

Purification

Purification on EC 1.1.1.195 - cinnamyl-alcohol dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by affinity purification on a nickel column; by affinity purification on a nickel column; recombinant enzyme; recombinant enzyme
Ni-NTA column chromatography
-
nickel affinity column chromatography
-
recombinant enzyme from strain BL9 by DEAE ion exchange, Red-Sepharose, and gel filtration chromatography
-
recombinant GH2-encoded CAD from Escherichia coli
-
recombinant GST-fusion enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography; recombinant GST-fusion enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant isozyme CAD4 from Escherichia coli; recombinant isozyme CAD5 from Escherichia coli
two isoforms
-
var. Mandarin
-