1.1.1.195: cinnamyl-alcohol dehydrogenase

This is an abbreviated version, for detailed information about cinnamyl-alcohol dehydrogenase, go to the full flat file.

Reaction

cinnamyl alcohol
+
NADP+
=
cinnamaldehyde
+
NADPH
+
H+

Synonyms

ADH, AdhA, alcohol dehydrogenase, Bmr6, brown midrib6, Brown-midrib 1 protein, CAD, CAD 7/8, CAD1, CAD10, CAD11, CAD12, CAD13, CAD14, CAD15, CAD2, CAD3, CAD4, CAD5, CAD6, CAD7, CAD8, CAD9, CADH I, cinnamyl alcohol dehydrogenase, cinnamyl alcohol dehydrogenase 1, cinnamyl alcohol dehydrogenase 2, dehydrogenase, cinnamyl alcohol, FC1, FLEXIBLE CULM1, More, ScAdh6p

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.195 cinnamyl-alcohol dehydrogenase

Cloned

Cloned on EC 1.1.1.195 - cinnamyl-alcohol dehydrogenase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
1.3 kb cDNA fragment cloned into pGEM-T Easy vector and transformed into competent cell of DH5alpha from Escherichia coli
9 AtCAD genes, 2 paralogs AtCAD-C and AtCAD-D, DNA and amino acid sequence determination and analysis, tissue expression pattern study of wild-type and mutant plants
construct pCFC1 containing the entire 8.8 kb open reading frame, 3253 bp upstream sequences, and the 1646 bp downstream region and an empty pCAMBIA2301 vector introduced into the fc1 mutant by Agrobacterium tumefaciens-mediated transformation
entire coding regions of CAD4 cloned into the pET30a vector and expressed in Escherichia coli Rossetta R2 cells; entire coding regions of CAD4 cloned into the pET30a vector and expressed in Escherichia coli Rossetta R2 cells; expression in Escherichia coli; expression in Escherichia coli
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
expression in Escherichia coli; expression in Escherichia coli; expression in Escherichia coli
from an expression sequence tag (EST) library, amplified fragments ligated into TOPO cloning vector. EST fragments in an entry vector cloned into the RNAi destination vector pIPKTA30N
-
gene CAD or HP1140 from strain 26995, cloning in Escherichia coli strain DH5alpha, and expression in strain BL21(DE3) as N-terminally His-tagged enzyme
-
gene CAD, DNA and amino acid sequence determination of several CAD isozyme genes, expression analysis in wild-type and cad-c-cad-d-double mutant cells, overview
gene CAD, genetic organization, DNA and amino acid sequence determination
-
gene CAD1, DNA and amino acid sequence determination and analysis, phylogenetic tree of the CAD protein family, quantitative expression analysis, expression in Escherichia coli, complementation of the cad-c-cad-d-double mutant with different CAD genes has different effects on lignin monomer synthesis, overview
gene CAD1-1, DNA and amino acid sequence determination and analysis, expression analysis, recombinant expression as GST-fusion enzyme in Escherichia coli strain BL21; gene CAD1-7, DNA and amino acid sequence determination and analysis, expression analysis, recombinant expression as GST-fusion enzyme in Escherichia coli strain BL21
gene Gold-hull-and-internode2, i.e. gene GH2, DNA and amino acid sequence determination and analysis, map-based cloning approach, phylogenetic and expression pattern analysis, expression of GH2 in Escherichia coli
-
genes Fxcad1 and Fxcad2, DNA and amino acid sequence determination and analysis, expression of gene Fxcad1 in Escherichia coli and in Pichia pastoris, in the latter case as an extracellular protein
genomic fragment giving the CAD promoter (829 bp) amplified
-
isozyme CAD4, expression in Escherichia coli; isozyme CAD5, expression in Escherichia coli
overexpression in strain BL9
-
partial DNA and amino acid sequence determination and analysis
Picea glauca seedlings line Pg653 stably transformed with a DNA fragment of 1163 base pairs (CAD) fused to the beta-glucuronidase gene