1.1.1.195: cinnamyl-alcohol dehydrogenase

This is an abbreviated version, for detailed information about cinnamyl-alcohol dehydrogenase, go to the full flat file.

Reaction

cinnamyl alcohol
+
NADP+
=
cinnamaldehyde
+
NADPH
+
H+

Synonyms

ADH, AdhA, alcohol dehydrogenase, AtCAD4, BdCAD1, Bmr6, Bradi3g06480, brown midrib6, Brown-midrib 1 protein, CAD, CAD 7/8, CAD1, CAD10, CAD11, CAD12, CAD13, CAD14, CAD15, CAD2, CAD3, CAD4, CAD5, CAD6, CAD7, CAD8, CAD9, CADH I, cinnamyl alcohol dehydrogenase, cinnamyl alcohol dehydrogenase 1, cinnamyl alcohol dehydrogenase 12, cinnamyl alcohol dehydrogenase 2, cinnamyl alcohol dehydrogenase 3, cinnamyl alcohol dehydrogenase 4, cinnamyl alcohol dehydrogenase 5, cinnamyl alcohol dehydrogenase 7, cinnamyl alcohol dehydrogenase 9, cinnamyl alcohol dehydrogenase C, cinnamyl alcohol dehydrogenases, CtCAD1, CtCAD2, CtCAD3, dehydrogenase, cinnamyl alcohol, FC1, FLEXIBLE CULM1, HcCAD1, HcCAD2, LlCAD2, LtuCAD1, More, Mt-CAD1, Mt-CAD2, PhCAD1, PhCAD2, PhCAD3, PhCAD4, PtoCAD1, PtoCAD12, PtoCAD2, PtoCAD3, PtoCAD5, PtoCAD6, PtoCAD7, PtoCAD8, PtoCAD9, ScAdh6p, TaCAD12

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.195 cinnamyl-alcohol dehydrogenase

Cloned

Cloned on EC 1.1.1.195 - cinnamyl-alcohol dehydrogenase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
1.3 kb cDNA fragment cloned into pGEM-T Easy vector and transformed into competent cell of DH5alpha from Escherichia coli
9 AtCAD genes, 2 paralogs AtCAD-C and AtCAD-D, DNA and amino acid sequence determination and analysis, tissue expression pattern study of wild-type and mutant plants
construct pCFC1 containing the entire 8.8 kb open reading frame, 3253 bp upstream sequences, and the 1646 bp downstream region and an empty pCAMBIA2301 vector introduced into the fc1 mutant by Agrobacterium tumefaciens-mediated transformation
diverse CAD or CAD-like genes in Medicago trunculata, phylogenetic analysis, recombinant expression of His-tagged Mt-CAD1 wild-type in Escherichia coli strain Rosetta2; diverse CAD or CAD-like genes in Medicago trunculata, phylogenetic analysis, recombinant expression of His-tagged Mt-CAD2 wild-type and mutants in Escherichia coli strain Rosetta2
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entire coding regions of CAD4 cloned into the pET30a vector and expressed in Escherichia coli Rossetta R2 cells; entire coding regions of CAD4 cloned into the pET30a vector and expressed in Escherichia coli Rossetta R2 cells; expression in Escherichia coli; expression in Escherichia coli
expressed in Escherichia coli BL21 cells; gene TaCAD12, cloned from cv. CI12633, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of GST-tagged enzyme, overexpression of the enyzem and of GFP-tagged enzyme in Triticum aestivum cv. CI12633
expressed in Escherichia coli BL21(DE3) cells; gene cad1, DNA and amino acid sequence determination and analysis, MtCAD1 is obtained by reverse genetic screening, genotying, RT-PCR analysis of CAD1 transcripts in cad1-1 and cad1-2 mutant and wild-type lines. The coding sequence of CAD1 driven by the 35S promoter is used for complementation of the cad1-1 mutant. The lignin level in the mutant is significantly restored, and the indene signature disappears, in six independent transformation events. MtCAD1 can also rescue the phenotype of the Arabidopsis thaliana cad4/cad5 double knockout mutant, in which the red coloration in the fibers of the double mutant is no longer visible in the complemented line, and acetyl bromide lignin levels are also partially rescued
expressed in Escherichia coli BL21(DE3) cells; gene PhCAD1, DNA and amino acid sequence determination and analysis, genotyping and phylogenetic analysis and transcriptome analysis, semi-quantitative RT-PCR enzyme expression analysis, overview. Recombinant expression of His-tagged enzyme; gene PhCAD2, DNA and amino acid sequence determination and analysis, genotyping and phylogenetic analysis and transcriptome analysis, semi-quantitative RT-PCR enzyme expression analysis, overview. Recombinant expression of His-tagged enzyme; gene PhCAD3, DNA and amino acid sequence determination and analysis, genotyping and phylogenetic analysis and transcriptome analysis, semi-quantitative RT-PCR enzyme expression analysis, overview. Recombinant expression of His-tagged enzyme; gene PhCAD4, DNA and amino acid sequence determination and analysis, genotyping and phylogenetic analysis and transcriptome analysis, semi-quantitative RT-PCR enzyme expression analysis, overview. Recombinant expression of His-tagged enzyme
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expression in Escherichia coli
expression in Escherichia coli; expression in Escherichia coli; expression in Escherichia coli
from an expression sequence tag (EST) library, amplified fragments ligated into TOPO cloning vector. EST fragments in an entry vector cloned into the RNAi destination vector pIPKTA30N
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gene BdCAD1, DNA and amino acid sequence determination of wild-type and mutant genes, genotyping, phylogenetic analysis and tree, quantitative RT-PCR expression analysis, functional complementation of the cad1 Bd4179 mutant with the wild-type BdCAD1 allele
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gene CAD or HP1140 from strain 26995, cloning in Escherichia coli strain DH5alpha, and expression in strain BL21(DE3) as N-terminally His-tagged enzyme
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gene CAD, DNA and amino acid sequence determination of several CAD isozyme genes, expression analysis in wild-type and cad-c-cad-d-double mutant cells, overview
gene CAD, genetic organization, DNA and amino acid sequence determination
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gene CAD, isozyme genotyping, phylogenetic analysis, sequence comparisons, and expression analysis of isozymes
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gene CAD, phylogenetic analysis and tree
gene CAD, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3)
gene CAD1, DNA and amino acid sequence determination and analysis, genetic localization CM3.5_scaffold00038, intron-exon structure of melon CAD genes, promoter sequence analysis, phylogenetic analysis, semi-quantitative PCR and quantitative real-time PCR expression analysis; gene CAD2, DNA and amino acid sequence determination and analysis, genetic localization CM3.5_scaffold00034, intron-exon structure of melon CAD genes, promoter sequence analysis, phylogenetic analysis, semi-quantitative PCR and quantitative real time PCR expression analysis; gene CAD3, DNA and amino acid sequence determination and analysis, genetic localization CM3.5_scaffold01596, intron-exon structure of melon CAD genes, promoter sequence analysis, phylogenetic analysis, semi-quantitative PCR and quantitative real time PCR expression analysis; gene CAD4, DNA and amino acid sequence determination and analysis, genetic localization CM3.5_scaffold00005, intron-exon structure of melon CAD genes, promoter sequence analysis, phylogenetic analysis, semi-quantitative PCR and quantitative real time PCR expression analysis; gene CAD5, DNA and amino acid sequence determination and analysis, genetic localization CM3.5_scaffold00059, intron-exon structure of melon CAD genes, promoter sequence analysis, phylogenetic analysis, semi-quantitative PCR and quantitative real time PCR expression analysis
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gene cad1, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree; gene cad2, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21
gene CAD1, DNA and amino acid sequence determination and analysis, phylogenetic tree of the CAD protein family, quantitative expression analysis, expression in Escherichia coli, complementation of the cad-c-cad-d-double mutant with different CAD genes has different effects on lignin monomer synthesis, overview
gene cad1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged PtoCAD1 in Escherichia coli strain M15; gene cad1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged PtoCAD1 in Escherichia coli strain M15; gene cad1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged PtoCAD1 in Escherichia coli strain M15; gene cad1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged PtoCAD1 in Escherichia coli strain M15; gene cad2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged PtoCAD1 in Escherichia coli strain M15; gene cad3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged PtoCAD3 in Escherichia coli strain M15; gene cad5, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged PtoCAD5 in Escherichia coli strain M15; gene cad7, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged PtoCAD7 in Escherichia coli strain M15; gene cad9, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged PtoCAD9 in Escherichia coli strain M15
gene CAD1-1, DNA and amino acid sequence determination and analysis, expression analysis, recombinant expression as GST-fusion enzyme in Escherichia coli strain BL21; gene CAD1-7, DNA and amino acid sequence determination and analysis, expression analysis, recombinant expression as GST-fusion enzyme in Escherichia coli strain BL21
gene CAD5 or Bradi3g06480, sequence comparisons of the CAD family enzymes from different species, phylogenetic tree
gene ctcad1, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative realtime PCR enzyme expression analysis; gene ctcad2, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative realtime PCR enzyme expression analysis; quantitative realtime PCR enzyme expression analysis during seed development, overview. CAD2 shows the highest expression level of all three CAD isozymes in Carthamus tinctorius
gene Gold-hull-and-internode2, i.e. gene GH2, DNA and amino acid sequence determination and analysis, map-based cloning approach, phylogenetic and expression pattern analysis, expression of GH2 in Escherichia coli
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gene LtuCAD1, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, when expressed in the Arabidopsis cad4/cad5 double mutant, LtuCAD1 is able to restore the total lignin content and decrease the S/G lignin ratio, recombinant expressionof the CAD1 gene driven by a CaMV 35S promoter in Arabidopsis thaliana Col-0 using the Agrobacterium tumefaciens strain GV13001 for transfection
gene PaCAD1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3); gene PaCAD2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
genes Fxcad1 and Fxcad2, DNA and amino acid sequence determination and analysis, expression of gene Fxcad1 in Escherichia coli and in Pichia pastoris, in the latter case as an extracellular protein
genomic fragment giving the CAD promoter (829 bp) amplified
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isozyme CAD4, expression in Escherichia coli; isozyme CAD5, expression in Escherichia coli
overexpression in strain BL9
-
partial DNA and amino acid sequence determination and analysis
Picea glauca seedlings line Pg653 stably transformed with a DNA fragment of 1163 base pairs (CAD) fused to the beta-glucuronidase gene