1.1.1.188: prostaglandin-F synthase

This is an abbreviated version, for detailed information about prostaglandin-F synthase, go to the full flat file.

Reaction

(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate
+
NADP+
=
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
+
NADPH
+
H+

Synonyms

20alpha-HSD/PGFS, 20alpha-hydroxysteroid dehydrogenase/prostaglandin F-synthase, AKR1B1, AKR1B3, AKR1B7, AKR1C3, aldo-keto reductase, aldo-keto reductase 1C3, ALDR1, ALR2, DD1, dihydrodiol dehydrogenase 1, m-PGFS, More, NADPH-dependent prostaglandin D2 11-keto reductase, PG endoperoxide reductase, PGD 11-ketoreductase, PGD2 11-ketoreductase, PGD2 11-ketoreductase activity, PGF synthase, PGF synthase I, PGF synthase II, PGF2alpha synthase, PGF2alpha-synthetic activity, PGFS, PGFS I, PGFS-I, PGFS-II, PGFSI, PGFSII, PGH 9-,11-endoperoxide reductase, PGH2 9,11-endoperoxidase, PGH2 9,11-endoperoxide reductase, PGH2 9-,11-endoperoxide reductase, prostaglandin 11-keto reductase, prostaglandin 11-ketoreductase, prostaglandin D2 11-ketoreductase, prostaglandin D2-ketoreductase, prostaglandin F synthase, prostaglandin F synthase I, prostaglandin F synthase II, prostaglandin F synthetase, prostaglandin F2alpha synthase, prostaglandin F2alpha-synthase, prostaglandin-D2 11-reductase, prostaglandin-F2alpha synthase, prostamide/PGF synthase, prostamide/prostaglandin F synthase, reductase, 15-hydroxy-11-oxoprostaglandin, synthetase, prostaglandin F2alpha, type 5 17beta-hydroxysteroid dehydrogenase/prostaglandin F synthase, vas deferens protein

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.188 prostaglandin-F synthase

Purification

Purification on EC 1.1.1.188 - prostaglandin-F synthase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
; recombinant enzyme from Escherichia coli strain HB101 to homogeneity
-
; recombinant enzyme from Escherichia coli, to homogeneity
-
enzyme from lung, to homogeneity
-
native liver-type isozyme from liver, 342fold to homogeneity by ammonium sulfate fractionation, anion exchange and adsorption chromatography
-
on Ni2+ column, 102- and 152fold purification of the prostamide F synthase and PGF synthase activities, respectively, achieved from the swine brain (90 g) with a yield of 1.4 and 2%, respectively. Mutants expressed to homogeneity
partial
-
recombinant enzyme from Escherichia coli by GSH affinity chromatography, cleavage of the fusion protein glutathione S-tranferase by thrombin, and DEAE ion exchange chromatography
-
recombinant enzyme from Escherichia coli to homogeneity
-
recombinant wild-type liver-type isoyzme and mutant enzymes from COS-7 cells, to homogeneity
-
to apparent homogeneity on Ni2+ column
-
to apparent homogeneity, on Ni-NTA resin and by gel filtration
-
to apparent homogeneity, on Ni-NTA resin and by gel filtration; to apparent homogeneity, on Ni-NTA resin and by gel filtration
-