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1.1.1.183: geraniol dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about geraniol dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.1.1.183

Reaction

geraniol
+
NADP+
=
geranial
+
NADPH
+
H+

Synonyms

GDH, GeDH, GEDH1, geoA, geraniol dehydrogenase, geraniol dehydrogenase-like enzyme, geraniol-DH I, geraniol-DH II, More, NADP+-dependent geraniol dehydrogenase, OaGDH, YjgB, ZoGeDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.183 geraniol dehydrogenase (NADP+)

Purification

Purification on EC 1.1.1.183 - geraniol dehydrogenase (NADP+)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate and 2-propanol fractionation
-
DEAE Toyopearl 650M column chromatography, phenyl Toyopearl 650 M column chromatography, and AF-Red Toyopearl 650ML column chromatography
-
native enzyme 1879fold to homogeneity by ammonium sulfate fractionation, followed by hydrophobic interactin and adsorption chromatography, anion exchange chromatography, and gel filtration in seven purification steps, overview
partially, ammonium sulfate fractionation, gel filtration, anion exchange chromatography
Citrus sp.
-
recombinant GEDH1 from Escherichia coli by anion exchange chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS