1.1.1.179: D-xylose 1-dehydrogenase (NADP+)

This is an abbreviated version, for detailed information about D-xylose 1-dehydrogenase (NADP+), go to the full flat file.

Reaction

D-xylose
+
NADP+
=
D-xylono-1,5-lactone
+
NADPH
+
H+

Synonyms

D-xylose (nicotinamide adenine dinucleotide phosphate) dehydrogenase, D-xylose dehydrogenase, D-xylose-NADP dehydrogenase, D-xylose:NADP+ oxidoreductase, NADP+-dependent D-xylose dehydrogenase, NADP+:D-xylose dehydrogenase, XDH, xyd1

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.179 D-xylose 1-dehydrogenase (NADP+)

Cloned

Cloned on EC 1.1.1.179 - D-xylose 1-dehydrogenase (NADP+)

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cDNA subcloned in expression vector pRset, overexpression in Escherichia coli
-
cloned into pET19b, resulting plasmid harvested from Escherichia coli JM109, sequenced, and transformed into Escherichia coli Rosetta(DE3)-pLysS expression strain; expression in Escherichia coli
expressed in Escherichia coli
-
expression in Kluyveromyces lactis
-
gene xyd1, DNA and amino acid sequence determination and analysis, functional expression of the His-tagged enzyme in Saccharomyces cerevisiae
A8BT09
overexpression in Escherichia coli
wild-type protein and mutants are expressed in Escherichia coli BL21 (DE3)