1.1.1.176: 12alpha-hydroxysteroid dehydrogenase

This is an abbreviated version, for detailed information about 12alpha-hydroxysteroid dehydrogenase, go to the full flat file.

Reaction

cholate
+
NADP+
=
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oate
+
NADPH
+
H+

Synonyms

12alpha-HSDH, 12alpha-hydroxy steroid dehydrogenase, dehydrogenase, 12alpha-hydroxy steroid, NAD-dependent 12alpha-hydroxysteroid dehydrogenase, NADP-12alpha-hydroxysteroid dehydrogenase, NADP-dependent 12alpha-hydroxysteroid dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.176 12alpha-hydroxysteroid dehydrogenase

Substrates Products

Substrates Products on EC 1.1.1.176 - 12alpha-hydroxysteroid dehydrogenase

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
12-ketochenodeoxycholic acid + NADH
cholic acid + NAD+
show the reaction diagram
-
-
-
r
12-oxochenodeoxycholic acid + NADP+
(3alpha,5beta,7alpha,12alpha)-3,7,12-trihydroxycholan-24-oic acid + NADPH + H+
show the reaction diagram
-
-
-
-
?
7alpha,12alpha-dihydroxy-5beta-cholanoate + NAD+
7alpha-hydroxy-12-oxo-5beta-cholanoate + NADH
show the reaction diagram
-
-
-
-
?
allocholate + NAD+
3alpha,7alpha-dihydroxy-12-oxo-5alpha-cholan-24-oic acid + NADH
show the reaction diagram
-
-
-
-
?
allodeoxycholate + NAD+
3alpha-hydroxy-12-oxo-5alpha-cholan-24-oic acid + NADH
show the reaction diagram
-
-
-
-
?
cholic acid + NAD(P)+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H
show the reaction diagram
cholic acid + NADP+
12-ketochenodeoxycholic acid + NADPH
show the reaction diagram
-
the assay is performed using an NADP+-regenerating system with acetone, NADPH, 2-propanol and alcohol dehydrogenase from Lactobacillus brevis or Thermoanaerobium brockii, overview
-
-
r
deoxycholic acid + NAD(P)+
3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+
show the reaction diagram
deoxycholic acid + NAD+
3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
glycocholic acid + NAD+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH
show the reaction diagram
glycodeoxycholic acid + NAD+
3alpha-hydroxy-12-oxo-5beta-cholanoyl glycine + NADH
show the reaction diagram
taurocholic acid + NAD(P)+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl taurine + NAD(P)H
show the reaction diagram
taurodeoxycholic acid + NAD+
3alpha-hydroxy-12-oxo-5beta-cholanoyl taurine + NADH
show the reaction diagram