1.1.1.176: 12alpha-hydroxysteroid dehydrogenase

This is an abbreviated version, for detailed information about 12alpha-hydroxysteroid dehydrogenase, go to the full flat file.

Reaction

cholate
+
NADP+
=
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oate
+
NADPH
+
H+

Synonyms

12alpha-HSDH, 12alpha-hydroxy steroid dehydrogenase, dehydrogenase, 12alpha-hydroxy steroid, NAD-dependent 12alpha-hydroxysteroid dehydrogenase, NADP-12alpha-hydroxysteroid dehydrogenase, NADP-dependent 12alpha-hydroxysteroid dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.176 12alpha-hydroxysteroid dehydrogenase

Storage Stability

Storage Stability on EC 1.1.1.176 - 12alpha-hydroxysteroid dehydrogenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 20 days, 50% loss of activity
-
-20°C, 3 months, 5% loss of activity
-
-20°C, lyophilized, 4 months
-
-30°C, 6 months, 50% glycerol, no loss of activity
-
-30°C, concentrated form, at least 6 months
-
4°C, 0.5 ml TEA-buffer, 3 days
-