1.1.1.176: 12alpha-hydroxysteroid dehydrogenase

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For detailed information about 12alpha-hydroxysteroid dehydrogenase, go to the full flat file.

Reaction

Synonyms

12alpha-HSDH, 12alpha-hydroxy steroid dehydrogenase, CDD59475, dehydrogenase, 12alpha-hydroxy steroid, Elen_2515, NAD+-dependent 12alpha-HSDH, NAD-dependent 12alpha-hydroxysteroid dehydrogenase, NADP+-dependent 12alpha-HSDH, NADP-12alpha-hydroxysteroid dehydrogenase, NADP-dependent 12alpha-hydroxysteroid dehydrogenase

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.176 12alpha-hydroxysteroid dehydrogenase

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Results	in table
4	AA Sequence
6	Application
1	CAS Registry Number
4	Cloned(Commentary)
26	Cofactor
3	General Information
1	General Stability
8	Inhibitors
16	kcat/KM [mM/s]
35	KM Value [mM]
9	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
31	Organism
2	Pathway
18	pH Optimum
1	pH Range
5	Protein Variants
11	Purification (Commentary)
1	Reaction
1	Reaction Type
30	Reference
1	Source Tissue
3	Specific Activity [micromol/min/mg]
6	Storage Stability
132	Substrates and Products (Substrate)
22	Subunits
31	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
6	Temperature Stability [°C]
16	Turnover Number [1/s]

Specific Activity

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Specific Activity on EC 1.1.1.176 - 12alpha-hydroxysteroid dehydrogenase

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0.027

Acinetobacter lwoffii

-

cell extract

701240

0.3

Acinetobacter lwoffii

-

purified enzyme

701240

additional information

Clostridium sp.

-

assay method development using an NADP+-regenerating system with acetone, NADPH, 2-propanol and alcohol dehydrogenase from Lactobacillus brevis or Thermoanaerobium brockii, optimization, overview

668040