1.1.1.17: mannitol-1-phosphate 5-dehydrogenase

This is an abbreviated version, for detailed information about mannitol-1-phosphate 5-dehydrogenase, go to the full flat file.

Reaction

D-mannitol 1-phosphate
+
NAD+
=
D-fructose 6-phosphate
+
NADH
+
H+

Synonyms

BbMPD, D-mannitol-1-phosphate dehydrogenase, dehydrogenase, mannitol 1-phosphate, fructose 6-phosphate reductase, hexose reductase, long-chain mannitol-1-phosphate 5-dehydrogenase, long-chain mannitol-1-phosphate dehydrogenase, M1PDH, M1PDH1, M1PDH2, M1PDH3, mannitol 1-phosphate 5-dehydrogenase, mannitol 1-phosphate dehydrogenase, mannitol-1-phosphate dehydrogenase, MPD, MpdA, MPDH, MtlD, NAD+-dependent mannitol-1-phosphate dehydrogenase, NADH-dependent mannitol-1-phosphate 5-dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.17 mannitol-1-phosphate 5-dehydrogenase

Purification

Purification on EC 1.1.1.17 - mannitol-1-phosphate 5-dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
precipitation techniques
-
recombinant enzyme
-
recombinant enzyme 2.1fold from Escherichia coli by affinity chromatography
recombinant His-tagged MtlD by nickel affinity chromatography
-
recombinant His6-tagged nezyme from Escherichia coli strain SG13009 by nickel affinity chromatography
-
to homogeneity, chromatography steps, affinity chromatography
-
to homogeneity, chromatography techniques
wild-type and mutant
-