1.1.1.159: 7alpha-hydroxysteroid dehydrogenase

This is an abbreviated version, for detailed information about 7alpha-hydroxysteroid dehydrogenase, go to the full flat file.

Reaction

cholate
+
NAD+
=
3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oate
+
NADH
+
H+

Synonyms

7-alpha-HSDH, 7-HSDH, 7alpha-HSD, 7alpha-HSDH, 7alpha-hydroxy steroid dehydrogenase, 7alpha-hydroxysteroid dehydrogenase, Bile acid-inducible protein, CA 7alpha-HSDH, Ca7alpha-HSDH, dehydrogenase, 7alpha-hydroxy steroid

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.159 7alpha-hydroxysteroid dehydrogenase

Purification

Purification on EC 1.1.1.159 - 7alpha-hydroxysteroid dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme 175fold by anion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction and affinity chromatography
-
partially purified by ion-exchange chromatography
-
recombinant enzyme from Escherichia coli by heat treatment at 55-60°C, polyethyleneimine treatment, ammonium sulfate fractionation, and gel filtration, followed by lyophilization
-
recombinant from Escherichia coli, high yield
-
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, anion exchange chromatography, and gel filtration
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS by metal affinity chromatography
wild-type and recombinant from Escherichia coli
-