1.1.1.159: 7alpha-hydroxysteroid dehydrogenase

This is an abbreviated version, for detailed information about 7alpha-hydroxysteroid dehydrogenase, go to the full flat file.

Reaction

cholate
+
NAD+
=
3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oate
+
NADH
+
H+

Synonyms

7-alpha-HSDH, 7-HSDH, 7alpha-HSD, 7alpha-HSDH, 7alpha-hydroxy steroid dehydrogenase, 7alpha-hydroxysteroid dehydrogenase, Bile acid-inducible protein, CA 7alpha-HSDH, Ca7alpha-HSDH, dehydrogenase, 7alpha-hydroxy steroid

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.159 7alpha-hydroxysteroid dehydrogenase

Engineering

Engineering on EC 1.1.1.159 - 7alpha-hydroxysteroid dehydrogenase

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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R16G
-
site-directed mutagenesis, kcat and Km of the R16G mutant increase by more than 4times and 5times compared with wild-type values, respectively, while the catalytic efficiency (kcat/Km) of R16G mutant decreases by 17.26% compared to the wild-type enzyme. The increase in Km indicates that affinity of R16G mutant toward NADP+ becomes weak, while the cofactor NADP(H) dissociates more easily from the binding site resulting in the increase in kcat
R194G
-
site-directed mutagenesis, the mutant shows slightly reduced catalytic efficiency compared with NADP+ compared to the wild-type enzyme
R38D
-
site-directed mutagenesis, inactive mutant
K163I
-
5.25% activity of wild-type activity
K163R
-
63.7% activity of wild-type activity
S146A
-
20.3% activity of wild-type activity
S146H
-
35.6% activity of wild-type activity
Y159F
-
no activity
Y159H
-
13.3% activity of wild-type activity
additional information