1.1.1.153: sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming)

This is an abbreviated version, for detailed information about sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming), go to the full flat file.

Reaction

L-erythro-7,8-dihydrobiopterin
+
NADP+
=
sepiapterin
+
NADPH
+
H+

Synonyms

AKR1B1, reductase, sepiapterin, sepiapterin reductase, SPR, SR

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.153 sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming)

Purification

Purification on EC 1.1.1.153 - sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming)

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
; native enzyme from erythrocytes
-
; native enzyme from erythrocytes by hydroxylapatite and NADP+-affinity chromatography, to homogeneity
-
brain, partially, from erythrocytes to homogeneity; native enzyme partially from brain by ammonium sulfate fractionation and hydroxylapatite chromatography, and from erythrocytes to homogeneity by affinity chromatography and isoelectric focusing
-
His GraviTrap nickel affinity column chromatography, gel filtration
-
liver, partially
-
liver; native enzyme 5000fold from liver by ammonium sulfate and protamine sulfate fractionation, and two times treatment by hydroxylapatite and anion exchange chromatography
-
Ni-NTA bead chromatography, MonoQ column chromatography, and Superdex 200 gel filtration
-
recombinant His-tagged native and selenomethionine-labeled enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, followed by gel filtration
-
recombinant protein of Escherichia coli
recombinant protein using His-tag