1.1.1.149: 20alpha-hydroxysteroid dehydrogenase

This is an abbreviated version, for detailed information about 20alpha-hydroxysteroid dehydrogenase, go to the full flat file.

Reaction

17alpha,20alpha-dihydroxypregn-4-en-3-one
+
NAD(P)+
=
17alpha-hydroxyprogesterone
+
NAD(P)H
+
H+

Synonyms

20-alpha-HSD, 20-alpha-hydroxysteroid dehydrogenase, 20alpha-HSD, 20alpha-HSDH, 20alpha-hydroxy steroid dehydrogenase, 20alpha-hydroxysteroid dehydrogenase, 20alpha-hydroxysteroid oxidoreductase, 20alpha-OH-SDH, AKR1C1, AKR1C23, AKR1C3, aldo-keto reductase family 1 member C1, HSD1

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.149 20alpha-hydroxysteroid dehydrogenase

Purification

Purification on EC 1.1.1.149 - 20alpha-hydroxysteroid dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity binding to glutathione-Sepharose and thrombin digestion of bacterial lysate, ion exchange and affinity chromatography of enzyme produced by insect cells
-
ammonium sulfate precipitation, affinity chromatography
-
ammonium sulfate precipitation, affinity chromatography, gel filtration
-
ammonium sulfate precipitation, chromatography
-
ammonium sulfate precipitation, ultrafiltration, chromatography
-
anion exchange and affinity chromatography
-
anion exchange and affinity chromatography, gel filtration, HPLC
-
co-purification with 17beta-estradiol dehydrogenase, EC 1.1.1.62, affinity chromatography
-
co-purification with aldehyde reductase and dihydrodiol dehydrogenase
-
column chromatography, gel filtration
-
five steps co-purification with aldehyde reductase and dihydrodiol dehydrogenase, ammonium sulfate precipitation, HPLC
-
gel filtration, affinity chromatography
-
ion exchange chromatography
-
isozyme HSD1 and HSD2, anion-exchange and affinity chromatography, capillary electrophoresis
-
partially by ammonium sulfate fractionation and chromatography
-
recombinant GST-fusion enzyme by glutahione affinity chromatography and cleavage of the GST-tag by Factor Xa, followed by ion exchange chromatography, and gel filtration, to homogeneity
six step procedure, ammonium sulfate fractionation, chromatography, gel filtration
-
solubilized, separated by SDS-PAGE, protein extraction from gel, renatured
-