1.1.1.149: 20alpha-hydroxysteroid dehydrogenase

This is an abbreviated version, for detailed information about 20alpha-hydroxysteroid dehydrogenase, go to the full flat file.

Reaction

17alpha,20alpha-dihydroxypregn-4-en-3-one
+
NAD(P)+
=
17alpha-hydroxyprogesterone
+
NAD(P)H
+
H+

Synonyms

20-alpha-HSD, 20-alpha-hydroxysteroid dehydrogenase, 20alpha-HSD, 20alpha-HSDH, 20alpha-hydroxy steroid dehydrogenase, 20alpha-hydroxysteroid dehydrogenase, 20alpha-hydroxysteroid oxidoreductase, 20alpha-OH-SDH, AKR1C1, AKR1C23, AKR1C3, aldo-keto reductase family 1 member C1, HSD1

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.149 20alpha-hydroxysteroid dehydrogenase

Engineering

Engineering on EC 1.1.1.149 - 20alpha-hydroxysteroid dehydrogenase

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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D309L
-
similar Km-value as wild-type
E127D
-
increase in Km-value
F311A
-
mutation results in increased Ki values for 3-chloro-5-phenylsalicylic acid and 3,5-dichlorosalicylic acid compared to the wild-type enzyme
F311L
-
mutation results in equal Ki value for 3-chloro-5-phenylsalicylic acid and increased Ki value for 3,5-dichlorosalicylic acid compared to the wild-type enzyme
H222I
-
dramatic increase in Km-value
L306
-
the mutation in AKR1C1 results in a 46fold reduction in the potency of the inhibitor 3,5-dicholorosalicylic acid
L306A
-
mutation results in 81fold increase Ki values for 3-chloro-5-phenylsalicylic acid compared to the wild-type enzyme
R304L
-
dramatic increase in Km-value
T307V
-
similar Km-value as wild-type
Y305F
-
similar Km-value as wild-type
Y55F
-
no enzymic activity
additional information