1.1.1.145: 3beta-hydroxy-DELTA5-steroid dehydrogenase

This is an abbreviated version, for detailed information about 3beta-hydroxy-DELTA5-steroid dehydrogenase, go to the full flat file.

Reaction

a 3beta-hydroxy-DELTA5-steroid
+
NAD+
=
a 3-oxo-DELTA5-steroid
+
NADH
+
H+

Synonyms

3-beta-hydroxy-5-ene steroid dehydrogenase, 3-beta-hydroxy-delta(5)-steroid dehydrogenase, 3beta hydroxysteroid dehydrogenase, 3beta-HSD, 3beta-HSD type II, 3beta-HSD1, 3beta-HSD2, 3beta-HSD22, 3beta-HSDH, 3beta-HSD_1, 3beta-hydroxy steroid dehydrogenase/5-ene-4-ene isomerase, 3beta-hydroxy steroid dehydrogenase/isomerase, 3beta-hydroxy-5-ene steroid dehydrogenase, 3beta-hydroxy-5-ene-steroid dehydrogenase, 3beta-hydroxy-5-ene-steroid oxidoreductase, 3beta-hydroxy-DELTA5-C27-steroid dehydrogenase/isomerase, 3beta-hydroxy-DELTA5-C27-steroid oxidoreductase, 3beta-hydroxysteroid dehydrogenase, 3beta-hydroxysteroid dehydrogenase 1, 3beta-hydroxysteroid dehydrogenase type 1, 3beta-hydroxysteroid dehydrogenase type 2, 3beta-hydroxysteroid dehydrogenase type II, 3beta-hydroxysteroid dehydrogenase type1, 3beta-hydroxysteroid dehydrogenase/DELTA5-DELAT4 isomerase type 2, 3beta-hydroxysteroid dehydrogenase/DELTA5-DELTA4 isomerase, 3beta-hydroxysteroid dehydrogenase/DELTA5-DELTA4-isomerase, 3beta-hydroxysteroid dehydrogenase/DELTA5-DELTA4-isomerase type II, 3beta-hydroxysteroid dehydrogenase/isomerase, 3beta-hydroxysteroid dehydrogenase/isomerase type 1, 3betaHSD, 3betaHSD1, 3betaHSD2, 5-ene-3-beta-hydroxysteroid dehydrogenase, 5alpha-dihydrotestosterone 3beta-hydroxysteroid dehydrogenase, dehydrogenase, 3beta-hydroxy-DELTA5-steroid, DELTA5-3beta-hydroxysteroid dehydrogenase, DHRS4, HSD3B, HSD3B2, HSDB1, More, progesterone reductase, steroid-DELTA5-3beta-ol dehydrogenase, type 1 3beta-hydroxysteroid dehydrogenase, type 1 3beta-hydroxysteroid dehydrogenase/isomerase, type 2 3beta-hydroxysteroid dehydrogenase, type 2 3beta-hydroxysteroid dehydrogenase/isomerase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.145 3beta-hydroxy-DELTA5-steroid dehydrogenase

Cloned

Cloned on EC 1.1.1.145 - 3beta-hydroxy-DELTA5-steroid dehydrogenase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloning of the cDNA encoding 3beta-HSD from an adrenal library, DNA and amino acid sequence determination and analysis, phylogenetic analysis
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DNA and amino acid sequence determination and analysis, tissue-specific expression analysis
DNA and amino acid sequence determination and analysis, transient and stable expression of the virus enzyme in Epithelioma papulosum cyprini cells, expression as EGFP-fusion protein exclusively in host cell mitochondria, enzyme overexpression does not influence viral replication or cell growth, but does suppress virus-induced cytopathic effects
expressed in MA-10 cells; gene HSD3B2, analysis of expression and its regulation involving Nur77 factors in H295R and MA-10 cell lines, overview
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expressed in Sf9 cells using the baculovirus system
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expressed in Sf9 cells using the baculovirus system; expressed in Sf9 cells using the baculovirus system
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expressed in Sf9 cells using the baculovirus system; expression in baculoviral system
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expressed in Sf9 insect cells
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expression analysis in abdominal aorta of 28 samples from japanese patients
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expression analysis of the enzyme in sciatic nerves
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expression in 293 human fetal kidney cells
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expression in COS-1 monkey kidney tumor cells
expression in Escherichia coli, His-tag
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expression in Mycobacterium smegmatis
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expression in Sf9 cells
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expression in Sf9 cells; expression in Sf9 insect cells
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expression in Sf9 cells; expression of soluble wild-type and mutant isozyme 3beta-HSD1 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system; expression of soluble wild-type and mutant isozyme 3beta-HSD2 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system
expression in Sf9 cells; expression of the isozyme type II in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system
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expression in Sf9 insect cells
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expression in Spodoptera fungiperda insect cells
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expression of 3beta HSD I and III in COS-1 cells
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expression of 3beta HSD I, III, IV, V or VI in COS-7 cells
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expression of soluble wild-type and mutant isozyme 3beta-HSD1 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system; expression of soluble wild-type and mutant isozyme 3beta-HSD2 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system
overexpression with his6-tag, using a baculovirus expression system
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transient expression of HSD I and II cDNA in HeLa cells
transient functional expression in HEK293T cells
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