1.1.1.140: sorbitol-6-phosphate 2-dehydrogenase

This is an abbreviated version, for detailed information about sorbitol-6-phosphate 2-dehydrogenase, go to the full flat file.

Reaction

D-sorbitol 6-phosphate
+
NAD+
=
D-fructose 6-phosphate
+
NADH
+
H+

Synonyms

D-glucitol-6-phosphate dehydrogenase, D-sorbitol-6-phosphate 2-dehydrogenase, D-sorbitol-6-phosphate dehydrogenase, dehydrogenase, D-sorbitol 6-phosphate, Glucitol-6-phosphate dehydrogenase, GutF, GutM, GutR, ketosephosphate reductase, MdS6PDH, More, operon gutFRMCBA, S6PDH, sorbitol-6-P-dehydrogenase, sorbitol-6-phosphate dehydrogenase, Stl6PDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.140 sorbitol-6-phosphate 2-dehydrogenase

Purification

Purification on EC 1.1.1.140 - sorbitol-6-phosphate 2-dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
176.5 fold purification
-
approximately 100 fold purification
-
GutR and GutM purified on Ni-NTA column
-
recombinant enzyme from strain BL21 by ammonium sulfate fractionation and gel filtration
-
similar enzyme also named sorbitol-6-phosphate dehydrogenase from Eriobotrya japonica
-
using Ni-NTA chromatography
-