1.1.1.14: L-iditol 2-dehydrogenase

This is an abbreviated version, for detailed information about L-iditol 2-dehydrogenase, go to the full flat file.

Reaction

L-Iditol
+
NAD+
=
L-sorbose
+
NADH
+
H+

Synonyms

ADH, D-sorbitol dehydrogenase, dehydrogenase, L-iditol, Dgeo_2865, glucitol dehydrogenase, Hxt13, Hxt15, Hxt16, Hxt17, L-iditol 2-dehydrogenase, L-iditol dehydrogenase (sorbitol), L-iditol:NAD oxidoreductase, L-iditol:NAD+ 5-oxidoreductase, LeSDH, MdSDH5, More, NAD+-dependent sorbitol dehydrogenase, NAD-dependent polyol dehydrogenase, NAD-dependent sorbitol dehydrogenase, NAD-SDH, NAD-sorbitol dehydrogenase, PDH-11300, polyol dehydrogenase, Protein tms1, SDH, SldA, SLDH, Sor1, Sor2, sorbitol dehydrogenase, sorbitol transporter, SORD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.14 L-iditol 2-dehydrogenase

Storage Stability

Storage Stability on EC 1.1.1.14 - L-iditol 2-dehydrogenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18°C, 2 days, non-purified enzyme in serum
-
-20°C, 70% loss of activity after 1 week, 0.1 mg/ml bovine serum albumin stabilizes
-
-20°C, purified recombinant enzyme, 3d, almost complete loss of activity
-20°C, stable for 4 months
-
-60°C, 5 mM 2-mercaptoethanol, several months
-
-85°C, good preservation
-
4°C several days
-
4°C, 2 mM dithiothreitol, 0.1 mM NADH, 15 days
-
4°C, 20 mM potassium phosphate buffer, pH 7.0, 3 months
-
immobilized enzyme loses 20% of activity after 10 days at room temperature
-