1.1.1.14: L-iditol 2-dehydrogenase

This is an abbreviated version, for detailed information about L-iditol 2-dehydrogenase, go to the full flat file.

Reaction

L-Iditol
+
NAD+
=
L-sorbose
+
NADH
+
H+

Synonyms

ADH, D-sorbitol dehydrogenase, dehydrogenase, L-iditol, Dgeo_2865, glucitol dehydrogenase, Hxt13, Hxt15, Hxt16, Hxt17, L-iditol 2-dehydrogenase, L-iditol dehydrogenase (sorbitol), L-iditol:NAD oxidoreductase, L-iditol:NAD+ 5-oxidoreductase, LeSDH, MdSDH5, More, NAD+-dependent sorbitol dehydrogenase, NAD-dependent polyol dehydrogenase, NAD-dependent sorbitol dehydrogenase, NAD-SDH, NAD-sorbitol dehydrogenase, PDH-11300, polyol dehydrogenase, Protein tms1, SDH, SldA, SLDH, Sor1, Sor2, sorbitol dehydrogenase, sorbitol transporter, SORD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.14 L-iditol 2-dehydrogenase

Purification

Purification on EC 1.1.1.14 - L-iditol 2-dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by solubilization with Triton X-100, DEAE-cellulose and -Sepharose, hydroxylapatite, and size exclusion chromatography, efficiency of different solubilization methods, overview
-
cobalt-based resin affinity chromatography
further purification from commercial preparation
-
recombinant from Escherichia coli by ammonium sulfate precipitation, anion-exchange and dye affinity cromatography, 23fold
-