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1.1.1.14: L-iditol 2-dehydrogenase

This is an abbreviated version!
For detailed information about L-iditol 2-dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.14

Reaction

L-Iditol
+
NAD+
=
L-sorbose
 +
NADH
 +
H+

Synonyms

ADH, D-sorbitol dehydrogenase, dehydrogenase, L-iditol, Dgeo_2865, glucitol dehydrogenase, GoSCR, L-iditol 2-dehydrogenase, L-iditol dehydrogenase (sorbitol), L-iditol:NAD oxidoreductase, L-iditol:NAD+ 5-oxidoreductase, LeSDH, MdSDH5, More, NAD+-dependent sorbitol dehydrogenase, NAD-dependent polyol dehydrogenase, NAD-dependent sorbitol dehydrogenase, NAD-SDH, NAD-sorbitol dehydrogenase, PDH-11300, polyol dehydrogenase, Protein tms1, SDH, SldA, SLDH, SOR, Sor1, Sor2, sorbitol dehydrogenase, sorbitol dehydrogenase 1, sorbitol dehydrogenase 2, sorbitol related enzyme, SORD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.14 L-iditol 2-dehydrogenase

Cloned

Cloned on EC 1.1.1.14 - L-iditol 2-dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, co-expression with gene sldB in Escherichia coli strain JM109, sldB and PQQ are required for recombinant activity in Escherichia coli, co-expression of sldB is also required for enzyme activity in vivo
-
electrophoretic karyotyping and array-based comparative genomic hybridization (array-CGH), comparison of four different species derived from the Saccharomyces sensu stricto complex of 22 distillery strains, overview. The genomic diversity is mainly revealed within subtelomeric regions and the losses and/or gains of fragments of chromosomes I, III, VI and IX are the most frequently observed. Statistically significant differences in the gene copy number are documented in six functional gene categories: 1. telomere maintenance via recombination, DNA helicase activity or DNA binding, 2. maltose metabolism process, glucose transmembrane transporter activity, 3. asparagine catabolism, cellular response to nitrogen starvation, localized in cell wall-bounded periplasmic space, 4. siderophore transport, 5. response to copper ion, cadmium ion binding and 6. L-iditol 2-dehydrogenase activity. Distillery yeasts are diploid. Gene ontology overrepresentation profiles are species-specific
expressed in Pseudomonas putida strain IFO3738 fused to 6 x His-tag
expression in Escherichia coli
expression in Escherichia coli strain BL21
-
expression of wild-type and mutant enzymes in Escherichia coli
-
gene GoSCR, recombinant expression of His-tagged enzyme GoSCR in Escherichia coli strain BL21 (DE3), coexpression with 2,3-butanediol dehydrogenase (BDHA) from Bacillus subtilis for asymmetric reduction of 2-hydroxyacetophenone (2-HAP) to (R)-1-phenyl-1,2-ethanediol ((R)-PED) or (S)-1-phenyl-1,2-ethanediol ((S)-PED)
gene MdSDH5, DNA and amino acid sequence determination and analysis, transient expression of MdSDH5-GFP and MdSDH6-GFP in the mesophyll protoplast of Arabidopsis thaliana
gene MdSDH6, DNA and amino acid sequence determination and analysis, phylogenetic tree, transient expression of MdSDH5-GFP and MdSDH6-GFP in the mesophyll protoplast of Arabidopsis thaliana
gene SDh, DNA and amino acid sequence determination and analysis, isolation, characterization and evaluation of the Pichia pastoris sorbitol dehydrogenase promoter for expression of heterologous proteins, recombinant expression of several different enzymes using the SDH promoter, overview
-
molecular karyotyping reveals the diversity of chromosome patterns, four strains with the most accented genetic variabilities are selected and subjected to genome-wide array-based comparativ genomic hybridization (array-CGH) analysis. The differences in the gene copy number are found in five functional gene categories: (1) maltose metabolism and transport, (2) response to toxin, (3) siderophore transport, (4) cellular aldehyde metabolic process, and (5) L-iditol 2-dehydrogenase activity. Genomic stability and nucleolus state are affected in Saflager W-34/70 strain
multiple genes, DNA sequence determination and analysis, a cDNA is expressed in Escherichia coli