1.1.1.138: mannitol 2-dehydrogenase (NADP+)

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For detailed information about mannitol 2-dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.1.1.138

1.1.1.138

spindle

checkpoint

d-fructose

kinetochore

anaphase

cladosporium

bub3

kinetochore-microtubule

1.1.1.67

herbarum

l-xylulose

d-arabinitol

gluconobacter

jecorina

mad3

ige-binding

hypocrea

unattached

synthesis

analysis

biotechnology

Reaction

Synonyms

Cla h 8, Cla h 8 allergen, D-mannitol 2-dehydrogenase, D-mannitol dehydrogenase, D-mannitol dehydrogenase, NADP-dependent, Gox1432, LRMDH, LXR1, MAD1p, mannitol 2-dehydrogenase, Mannitol 2-dehydrogenase [NADP+], mannitol dehydrogenase, mannitol dehydrogenase (NADP+), mannitol dehydrogenase (nicotinamide dinucleotide phosphate), mannitol dehydrogenase 1, MDH, More, MtDH, NADP+-dependent mannitol dehydrogenase, NADP-dependent mannitol dehydrogenase, NADP-dependent MtDH, NADP-mannitol dehydrogenase, PIG8p, PSLDR, TbMDH

ECTree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.1.138 mannitol 2-dehydrogenase (NADP+)

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Results	in table
5	Activating Compound
26	AA Sequence
6	Application
1	CAS Registry Number
11	Cloned(Commentary)
59	Cofactor
6	Crystallization (Commentary)
2	Expression
3	General Information
49	Inhibitors
3	kcat/KM [mM/s]
6	Ki Value [mM]
69	KM Value [mM]
1	Localization
12	Metals/Ions
31	Molecular Weight [Da]
47	Natural Substrates/ Products (Substrates)
56	Organism
2	Pathway
28	PDB ID
25	pH Optimum
5	pH Range
3	pH Stability
4	pI Value
4	Protein Variants
16	Purification (Commentary)
3	Reaction
3	Reaction Type
48	Reference
38	Source Tissue
22	Specific Activity [micromol/min/mg]
3	Storage Stability
104	Substrates and Products (Substrate)
21	Subunits
43	Synonyms
1	Systematic Name
10	Temperature Optimum [°C]
2	Temperature Range [°C]
6	Temperature Stability [°C]

Purification

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Purification on EC 1.1.1.138 - mannitol 2-dehydrogenase (NADP+)

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3 entries

native enzyme 13.4fold by ammonium sulfate fractionation, hydrophobic interaction chromatography, and anion exchange chromatography

Cladosporium herbarum

P0C0Y5

669463

native enzyme from mycelia 368fold by anion exchange chromatography, ultrafiltration, and 2',5'-ADP affinity chromatography

partial

purified by standard chromatographic methods

Cladosporium herbarum

P0C0Y5

669463

recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultratfiltration

Limosilactobacillus reuteri

667280

to crystalline state, chromatography techniques, crystallization

Gluconobacter oxydans

285855

to homogeneity, affinity chromatography

Agaricus bisporus

285865

to homogeneity, chromatography techniques

2 entries

to homogeneity, chromatography techniques, affinity chromatography

Aspergillus parasiticus

285861