1.1.1.138: mannitol 2-dehydrogenase (NADP+)

This is an abbreviated version, for detailed information about mannitol 2-dehydrogenase (NADP+), go to the full flat file.

Reaction

D-mannitol
+
NADP+
=
D-fructose
+
NADPH
+
H+

Synonyms

Cla h 8, Cla h 8 allergen, D-mannitol 2-dehydrogenase, D-mannitol dehydrogenase, D-mannitol dehydrogenase, NADP-dependent, LRMDH, LXR1, MAD1p, Mannitol 2-dehydrogenase [NADP+], mannitol dehydrogenase, mannitol dehydrogenase (NADP+), mannitol dehydrogenase (nicotinamide dinucleotide phosphate), mannitol dehydrogenase 1, MDH, More, MtDH, NADP-dependent mannitol dehydrogenase, NADP-dependent MtDH, NADP-mannitol dehydrogenase, PIG8p, PSLDR, TbMDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.138 mannitol 2-dehydrogenase (NADP+)

Purification

Purification on EC 1.1.1.138 - mannitol 2-dehydrogenase (NADP+)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme 13.4fold by ammonium sulfate fractionation, hydrophobic interaction chromatography, and anion exchange chromatography; purified by standard chromatographic methods
native enzyme from mycelia 368fold by anion exchange chromatography, ultrafiltration, and 2',5'-ADP affinity chromatography
partial
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultratfiltration
-
to crystalline state, chromatography techniques, crystallization
-
to homogeneity, affinity chromatography
-
to homogeneity, chromatography techniques
to homogeneity, chromatography techniques, affinity chromatography
-