1.1.1.138: mannitol 2-dehydrogenase (NADP+)

This is an abbreviated version, for detailed information about mannitol 2-dehydrogenase (NADP+), go to the full flat file.

Reaction

D-mannitol
+
NADP+
=
D-fructose
+
NADPH
+
H+

Synonyms

Cla h 8, Cla h 8 allergen, D-mannitol 2-dehydrogenase, D-mannitol dehydrogenase, D-mannitol dehydrogenase, NADP-dependent, LRMDH, LXR1, MAD1p, Mannitol 2-dehydrogenase [NADP+], mannitol dehydrogenase, mannitol dehydrogenase (NADP+), mannitol dehydrogenase (nicotinamide dinucleotide phosphate), mannitol dehydrogenase 1, MDH, More, MtDH, NADP-dependent mannitol dehydrogenase, NADP-dependent MtDH, NADP-mannitol dehydrogenase, PIG8p, PSLDR, TbMDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.138 mannitol 2-dehydrogenase (NADP+)

Cloned

Cloned on EC 1.1.1.138 - mannitol 2-dehydrogenase (NADP+)

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, the enzyme shows a carbon responsive transcriptional pattern and is up-regulated when mycelia are transferred to a culture medium containing D-mannitol or D-fructose, quantitative expression and phylogenetic analysis
expressed in E. coli; gene MtDH, DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
expressed in Escherichia coli
expression in Escherichia coli
-
expression in Pichia pastoris, gene cloning in Escherichia coli
-
expression of mutant enzymes in Escherichia coli strain JM109
-
gene MAD1, expression analysis in fungal tissue, recombinant expression in yeast
-
gene mdh, DNA and amino acid sequence determination and compiational amnipulation, expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-