1.1.1.133: dTDP-4-dehydrorhamnose reductase

This is an abbreviated version, for detailed information about dTDP-4-dehydrorhamnose reductase, go to the full flat file.

Reaction

dTDP-beta-L-rhamnose
+
NADP+
=
dTDP-4-dehydro-beta-L-rhamnose
+
NADPH
+
H+

Synonyms

4-ketoreductase, Agl14, bifunctional UDP-4-keto-6-deoxy-D-glucose epimerase/reductase, dTDP-4-dehydrorhamnose reductase, dTDP-4-keto-L-rhamnose reductase, dTDP-4-keto-rhamnose reductase, dTDP-4-ketorhamnose reductase, dTDP-6-deoxy-L-lyxo-4-hexulose reductase, dTDP-6-deoxy-L-mannose dehydrogenase, dTDP-L-rhamnose synthetase, gacA, RmlD, TDP-4-keto-rhamnose reductase, TDP-4-ketorhamnose reductase, thymidine diphosphate-4-dehydrorhamnose reductase, thymidine diphosphate-6-deoxy-L-talose, thymidine diphospho-4-ketorhamnose reductase, UGER

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.133 dTDP-4-dehydrorhamnose reductase

Crystallization

Crystallization on EC 1.1.1.133 - dTDP-4-dehydrorhamnose reductase

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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
apo-enzyme, and in complex with NADH, NADPH or NADPH/dTDP-L-rhamnose
-
sitting drop vapour-diffusion method, 6 d
-
hanging drop vapor diffusion method, using 12.5% (w/v) PEG 1000, 12.5% (w/v) PEG 3350, 12.5% (v/v) 2-methyl-2,4-pentanediol, 0.02 M of each carboxylic acid (0.2 M sodium formate, 0.2 M ammonium acetate, 0.2 M trisodium citrate, 0.2 M sodium potassium L-tartrate, 0.2 M sodium oxamate) and 0.1 M MES/imidazole pH 6.5
-