1.1.1.132: GDP-mannose 6-dehydrogenase

This is an abbreviated version, for detailed information about GDP-mannose 6-dehydrogenase, go to the full flat file.

Reaction

GDP-D-mannose
+ 2 NAD+ +
H2O
=
GDP-D-mannuronate
+ 2 NADH + 2 H+

Synonyms

algD, GDP mannose dehydrogenase, GDP-mannose dehydrogenase, GMD, guanosine diphospho-D-mannose dehydrogenase, guanosine diphosphomannose dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.132 GDP-mannose 6-dehydrogenase

Purification

Purification on EC 1.1.1.132 - GDP-mannose 6-dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate fractionation, affinity chromatography
-
ammonium sulfate precipitation
-
pH/heat treatment acetone precipitation HPLC gel filtration chromatography
-
protamine sulfate fractionation
-
protamine sulfate precipitation, gel exclusion, ion exchange chromatography
-
recombinant wild-type and mutant enzymes from Escherichia coli by gel filtration of enzyme with tightly bound GDP-mannuronic acid and NAD+, dialysis and ion exchange chromatography
-
using Ni-NTA chromatography
-