18.104.22.168: GDP-mannose 6-dehydrogenase
This is an abbreviated version, for detailed information about GDP-mannose 6-dehydrogenase, go to the full flat file.
algD, GDP mannose dehydrogenase, GDP-mannose dehydrogenase, GMD, guanosine diphospho-D-mannose dehydrogenase, guanosine diphosphomannose dehydrogenase
Purification on EC 22.214.171.124 - GDP-mannose 6-dehydrogenase
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ammonium sulfate fractionation, affinity chromatography
ammonium sulfate precipitation
pH/heat treatment acetone precipitation HPLC gel filtration chromatography
protamine sulfate fractionation
protamine sulfate precipitation, gel exclusion, ion exchange chromatography
recombinant wild-type and mutant enzymes from Escherichia coli by gel filtration of enzyme with tightly bound GDP-mannuronic acid and NAD+, dialysis and ion exchange chromatography
using Ni-NTA chromatography