1.1.1.121: aldose 1-dehydrogenase (NAD+)

This is an abbreviated version, for detailed information about aldose 1-dehydrogenase (NAD+), go to the full flat file.

Reaction

D-aldose
+
NAD+
=
D-aldonolactone
+
NADH
+
H+

Synonyms

aldohexose dehydrogenase, aldose dehydrogenase, AldT, D-aldohexose dehydrogenase, dehydrogenase, D-aldohexose, More, Ta0754, TAD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.121 aldose 1-dehydrogenase (NAD+)

Temperature Stability

Temperature Stability on EC 1.1.1.121 - aldose 1-dehydrogenase (NAD+)

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
46
-
half-life: 1 min, biphasic inactivation curve
50
-
rapidly inactivated
55
-
half-life: 40 s in sodium phosphate buffer, pH 7.0, with D-fucose, D-galactose, D-glucose or D-mannose as substrate
60
-
stable up to, pH 7.5, 15 min
85
approximately 40% of the activity is inactivated by the incubation of the enzyme solution (0.3 mg/ml in 10 mM potassium phosphate, pH 7.0) for 15 min at 95C