1.1.1.12: L-arabinitol 4-dehydrogenase

This is an abbreviated version, for detailed information about L-arabinitol 4-dehydrogenase, go to the full flat file.

Reaction

L-arabinitol
+
NAD+
=
L-xylulose
+
NADH
+
H+

Synonyms

dehydrogenase, L-arabinitol, L-arabitol dehydrogenase, LAD, pentitol-DPN dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.12 L-arabinitol 4-dehydrogenase

Engineering

Engineering on EC 1.1.1.12 - L-arabinitol 4-dehydrogenase

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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D213S/I214R
-
significantly higher preference for NADP+ over NAD+
M70F
-
almost complete loss of activity; results in a nearly complete enzyme inactivation
Y318F
-
increased activity for L-arabinitol and xylitol, increased affinity for D-sorbitol; results in a significant increase in affinity for D-sorbitol, xylitol and L-arabitol
D211S
strong decrease in activity
D211S/I212R
strong decrease in activity, increase in activity with cofactor NADP+
D211S/I212R/D213N
strong decrease in activity, increase in activity with cofactor NADP+
D211S/I212R/S348T
strong decrease in activity, increase in activity with cofactor NADP+
D212S/I213R
D224S/I225R
-
significantly altered cofactor specificity from NAD+ to NADP+
additional information
-
gene disruption mutant, almost unable to grow on L-arabinose, extremely weak growth on L-arabinitol, D-talitol, galactitol