1.1.1.12: L-arabinitol 4-dehydrogenase

This is an abbreviated version, for detailed information about L-arabinitol 4-dehydrogenase, go to the full flat file.

Reaction

L-arabinitol
+
NAD+
=
L-xylulose
+
NADH
+
H+

Synonyms

dehydrogenase, L-arabinitol, HjLAD, L-arabinitol dehydrogenase, L-arabitol dehydrogenase, LAD, Lad1, LADA, pentitol-DPN dehydrogenase, XYL2

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.12 L-arabinitol 4-dehydrogenase

Cloned

Cloned on EC 1.1.1.12 - L-arabinitol 4-dehydrogenase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
derivatives of the expression vector pQE32 containing wild-type and mutated versions of ladA transformed to Escherichia coli M13 cells
-
expression in Escherichia coli
gene lad1, overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
gene lad1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene ladA, DNA and amino acid sequence determination and analysis; gene ladA, DNA and amino acid sequence determination and analysis, construction of strain 3M-43/pyrA, a pyrA mutant of strain 3M-43, in which an intact copy of ladA is inserted under control of the Aspergillus nidulans gpdA promoter. Complementation of the 3M-43 pyrA strain with pGpd:ladA
H6WCP4, JQ079782
gene xyl2, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain JM109, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)