glucose 1-dehydrogenase (NADP+)

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beta-D-glucose: NAD(P) 1-oxidoreductase, dehydrogenase, glucose (nicotinamide adenine dinucleotide phosphate), GDH, GlcDH, GlcDH 2, glucose dehydrogenase, halophilic glucose dehydrogenase, Hm GDH, NAD(P)+ glucose dehydrogenase, NADP-dependent glucose dehydrogenase, NADP-linked aldohexose dehydrogenase, nicotinamide adenine dinucleotide phosphate-linked aldohexose dehydrogenase, STK_17040


     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
       glucose 1-dehydrogenase (NADP+)


Crystallization on EC - glucose 1-dehydrogenase (NADP+)

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1.6 A structure determination of the enzyme using crystals grown under conditions that closely mimic those experienced within the cell of the halophile. The structure is compared with nonhalophilic homologues. Location of bound counterions in the structure. Analysis of protein-solvent interactions to further the understanding of the molecular basis of halophilic adaptation
; the structures of GlcDH in binary complexes with either NADP+/Zn2+ or NADPH/Zn2+, a ternary complex with NADP+/Zn2+/glucose, and a nonproductive ternary complex with NADP+/Zn2+ /gluconolactone are determined from isomorphous crystals, in the presence of zinc (1 mM) and either 5 mM NADP+, by using 1.4-1.6 M sodium citrate as the precipitant (in 100 mM HEPES, pH 7.0, and 2 M NaCl) or 5 mM NADPH, using 67-72% (w/v) 2-methyl-2,4-pentanediol as precipitant (in 100 mM HEPES, pH 7.5, and 2 M NaCl)
hanging-drop vapour diffusion; hanging-drop vapour-diffusion method at 17°C, crystallization of a range of binary and ternary complexes of the wild-type and a D38C mutant protein. For most of the complexes, crystals belonging to space group I222 are obtained using sodium/potassium citrate as a precipitant. For the binary and non-productive ternary complexes with NADPH/Zn, it is necessary to replace the citrate with 2-methyl-2,4-pentanediol. Despite the radical change in conditions, the crystals thus formed are isomorphous
hanging-drop vapour-diffusion method using sodium citrate as the precipitant. Two crystal forms representing the free enzyme and the binary complex with NADP+ grow under these conditions. Crystals of form I diffract to beyond 3.5 A resolution and belong to the hexagonal space group P622, with unit-cell parameters a = b = 89.1, c = 214.6 A, alpha = beta = 90°, gamma = 120°. Crystals of form II diffract to greater than 2.0 A and belong to the orthorhombic space group I222 or I212121, with unit-cell parameters a = 61.8, b = 110.9, c = 151.7 A, alpha = beta = gamma = 90°