1.1.1.119: glucose 1-dehydrogenase (NADP+)

This is an abbreviated version, for detailed information about glucose 1-dehydrogenase (NADP+), go to the full flat file.

Reaction

D-glucose
+
NADP+
=
D-glucono-1,5-lactone
+
NADPH
+
H+

Synonyms

beta-D-glucose: NAD(P) 1-oxidoreductase, dehydrogenase, glucose (nicotinamide adenine dinucleotide phosphate), GDH, GlcDH, GlcDH 2, glucose dehydrogenase, halophilic glucose dehydrogenase, Hm GDH, NAD(P)+ glucose dehydrogenase, NADP-dependent glucose dehydrogenase, NADP-linked aldohexose dehydrogenase, nicotinamide adenine dinucleotide phosphate-linked aldohexose dehydrogenase, STK_17040

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.119 glucose 1-dehydrogenase (NADP+)

Application

Application on EC 1.1.1.119 - glucose 1-dehydrogenase (NADP+)

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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
-
method for determination of D-glucose- and D-galactose levels in glycoconjugates. The NAD(P)H produced from the enzymatic oxidation of the monosaccharides reacts with a CuSO4-bathocuproinedisulfonic acid reagent to produce a color complex absorbing maximally at 486 nm. With galactose dehydrogenase and glucose dehydrogenase serving as the model enzymes, reaction analysis gives a linear plot from 2.5 to 250 nmol of sugar. Method has been applicated to sugar released by acid hydrolysis from lactose, porcine submaxillary mucin and raffinose was quantified
industry
-
Optimizing whole-cell biocatalysts by integrating a recombinant intracellular NADPH regeneration system through co-expression of a glucose facilitator from Zymomonas mobilis for uptake of unphosphorylated glucose and a NADP+-dependent glucose dehydrogenase from Bacillus megaterium that oxidizes glucose to gluconolactone.
synthesis