1.1.1.118: glucose 1-dehydrogenase (NAD+)

This is an abbreviated version, for detailed information about glucose 1-dehydrogenase (NAD+), go to the full flat file.

Reaction

D-glucose
+
NAD+
=
D-glucono-1,5-lactone
+
NADH
+
H+

Synonyms

D-aldohexose dehydrogenase, D-glucose:NAD oxidoreductase, dehydrogenase, glucose (nicotinamide adenine dinucleotide), GDH, GDH1, glucose dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.118 glucose 1-dehydrogenase (NAD+)

Engineering

Engineering on EC 1.1.1.118 - glucose 1-dehydrogenase (NAD+)

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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E170K
-
has enhanced stability when compared to the wild-type with half-life of ca. 9 min at 65°C. Stability dependence on salt concentration
E170K/Q252L
-
has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95
P45A/F155Y/V227A
-
has enhanced stability when compared to the wild-type with half-life of ca. 0.05 min at 65°C. Most labile variant has significantly lower stability in ethanol, acetone, and 1,4-dioxane than other variants
P45A/N46E/F155Y/E170K/V227A/W230F/Q252L
-
has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95
E170K
-
has enhanced stability when compared to the wild-type with half-life of ca. 9 min at 65°C. Stability dependence on salt concentration
-
E170K/Q252L
-
has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95
-
P45A/F155Y/V227A
-
has enhanced stability when compared to the wild-type with half-life of ca. 0.05 min at 65°C. Most labile variant has significantly lower stability in ethanol, acetone, and 1,4-dioxane than other variants
-
P45A/N46E/F155Y/E170K/V227A/W230F/Q252L
-
has enhanced stability when compared to the wild-type with half-life of ca. 5000 min at 65°C. The mutant demonstrates high stability regardless of salt-type at both water activities of 0.99 and 0.95
-