1.1.1.105: all-trans-retinol dehydrogenase (NAD+)

This is an abbreviated version, for detailed information about all-trans-retinol dehydrogenase (NAD+), go to the full flat file.

Reaction

all-trans-retinol-[cellular-retinol-binding-protein]
+
NAD+
=
all-trans-retinal-[cellular-retinol-binding-protein]
+
NADH
+
H+

Synonyms

all-trans retinol dehydrogenase, all-trans-retinol dehydrogenase, dehydrogenase, retinol, DHRS9, epidermal retinol dehydrogenase 2, LOC100185899, MDR, microsomal retinol dehydrogenase, mRDH1, NAD+-dependent retinoid-active short-chain dehydrogenase/reductase, P32, RDH-E2, RDH-like SDR, Rdh1, RDH10, RDH10a, RDH10b, RDH10c, RDH12, RDH16, RDH2, RDH7, RDH8, RDHE2, RDHE2S, RDHL, retinal reductase, retinene reductase, retinoid dehydrogenase/reductase, retinol dehydrogenase (vitamin A1), retinol dehydrogenase 10, retinol dehydrogenase 12, retinol dehydrogenase 2, retinol dehydrogenase 4, retinol dehydrogenase epidermal 2-similar, RL-HSD, RoDH-4, RoDH-like 3alpha-hydroxysteroid dehydrogenase, RoDH4, SDR16C5, SDR16C6, XP_2120364

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.105 all-trans-retinol dehydrogenase (NAD+)

Purification

Purification on EC 1.1.1.105 - all-trans-retinol dehydrogenase (NAD+)

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni2+-NTA resin column chromatography; recombinant enzyme
-
preparation of RoDH-4 contains a minor contaminating protein band. Therefore, the exact concentration of RoDH-4 is determined based on the calibration curve constructed from several concentrations of BSA loaded on to the same SDS/PAGE gel
-
RDH1 activity during purification is maintained only with phosphotidylcholine
-
solubilized enzyme is extremely unstabel and loses almost 80% of initial activity even at partially purified stage, purification uses resistance of enzyme to proteolysis at 4C in the presence of 1% Triton
-