1.1.1.100: 3-oxoacyl-[acyl-carrier-protein] reductase

This is an abbreviated version, for detailed information about 3-oxoacyl-[acyl-carrier-protein] reductase, go to the full flat file.

Reaction

a (3R)-3-hydroxyacyl-[acyl-carrier protein]
+
NADP+
=
a 3-oxoacyl-[acyl-carrier protein]
+
NADPH
+
H+

Synonyms

3-ketoacyl acyl carrier protein reductase, 3-ketoacyl-(acyl-carrier-protein) reductase, 3-ketoacyl-ACP reductase, 3-ketoacyl-ACP reductase/3R-hydroxyacyl-CoA dehydrogenase, 3-ketoacyl-ACP(CoA) reductase, 3-ketoacyl-acyl carrier protein reductase, 3-ketoacyl-thioester reductase, 3-ketoacyl-[acyl-carrier-protein] reductase, 3-oxo-acyl-ACP reductase, 3-oxoacyl-(acyl carrier protein) reductase, 3-oxoacyl-(acyl-carrier-protein) reductase, 3-oxoacyl-ACP reductase, 3-oxoacyl-AcpM reductase, 3-oxoacyl-thioester reductase, 3-oxoacyl-[ACP]reductase, ACP reductase, beta-ketoacyl acyl carrier protein (ACP) reductase, beta-ketoacyl acyl carrier protein reductase, beta-ketoacyl reductase, beta-ketoacyl thioester reductase, beta-ketoacyl-(acyl carrier protein) reductase, beta-ketoacyl-ACP reductase, beta-ketoacyl-acyl carrier protein reductase, beta-ketoacyl-[acyl carrier protein] reductase, beta-ketoacyl-[acyl-carrier protein] (ACP) reductase, BKR, FabG, FabG1, fabG4, KACPR, KAR, KCR1, KCR2, ketoacyl-acyl carrier protein reductase, MabA, MSMEG_6753, NADPH-dependent acetoacetyl coenzyme A reductase, NADPH-specific 3-oxoacyl-[acylcarrier protein]reductase, OAR, OAR1, Oar1p, PHA-specific acetoacetyl-CoA reductase, PhaB, polyhydroxyalkanoate-specific acetoacetyl coenzyme A reductase, reductase, 3-oxoacyl-[acyl carrier protein], RhlG

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.100 3-oxoacyl-[acyl-carrier-protein] reductase

Specific Activity

Specific Activity on EC 1.1.1.100 - 3-oxoacyl-[acyl-carrier-protein] reductase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0017 - 0.0018
-
recombinant refolded enzyme
0.0019
-
recombinant soluble enzyme
0.0132
-
-
0.0168
-
-
0.02
with 2-octanone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.0366
-
-
0.06
mutant enzyme K173A, with acetoacetyl-CoA and NADH, pH 7.4 and 25C
0.08
with 4'-fluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.13
with 3'-fluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0; with acetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.15
with 4'-chloroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.16
with propiophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
0.44
mutant enzyme R34A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25C; mutant enzyme R34A, with acetoacetyl-CoA and NADPH, pH 7.4 and 25C
0.46
mutant enzyme K152A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25C
0.5
mutant enzyme Q126E/R168E/K169E, with acetoacetyl-CoA and NADPH, pH 7.4 and 25C
0.65
mutant enzyme Y169A, with acetoacetyl-CoA and NADH, pH 7.4 and 25C
0.85
mutant enzyme Y169A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25C
1.03
with 1,1,1-trifluoroacetone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
1.16
with ethylbenzoylacetate as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
1.2
mutant enzyme K173A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25C
1.3
mutant enzyme K169E, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25C
1.6
mutant enzyme R168E, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25C
1.7
mutant enzyme Q126E/K169E, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25C
2.4
mutant enzyme K173A, with acetoacetyl-CoA and NADPH, pH 7.4 and 25C
2.45
with alpha-chloroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
2.6
wild type enzyme, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25C
3
-
mutant R187A/R230A, acyl-carrier protein-dependent spectroscopic assay
3.1
mutant enzyme Y169A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25C
3.5
wild type enzyme, with acetoacetyl-CoA and NADPH, pH 7.4 and 25C
3.93
with 2',3',4',5',6'-pentafluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
4
-
mutant R230E, acyl-carrier protein-dependent spectroscopic assay
4.3
mutant enzyme Q126E/K169E, with acetoacetyl-CoA and NADPH, pH 7.4 and 25C
4.5
mutant enzyme K173A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25C
5.4
mutant enzyme D42A, with 9,10-phenanthrene quinone and NADP+, pH 7.4 and 25C
5.5
mutant enzyme K169E, with acetoacetyl-CoA and NADPH, pH 7.4 and 25C
6.1
-
mutant R230A, acyl-carrier protein-dependent spectroscopic assay
6.9
-
mutant R187A, acyl-carrier protein-dependent spectroscopic assay
7.3
mutant enzyme D42A, with acetoacetyl-CoA and NADH, pH 7.4 and 25C
7.9
mutant enzyme R168E, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25C
8.4
mutant enzyme Q126E/R168E/K169E, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25C
14.8
mutant enzyme Q126E/R168E/K169E, with acetoacetyl-CoA and NADH, pH 7.4 and 25C
15.7
mutant enzyme K169E, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25C
17.4
mutant enzyme Q126E/K169E, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25C
19.3
mutant enzyme K169E, with acetoacetyl-CoA and NADH, pH 7.4 and 25C
20
mutant enzyme D42A, with acetoacetyl-CoA and NADPH, pH 7.4 and 25C
20.5
wild type enzyme, with acetoacetyl-CoA and NADH, pH 7.4 and 25C
20.7
mutant enzyme R34A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25C
20.8
wild type enzyme, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25C
21
mutant enzyme R34A, with acetoacetyl-CoA and NADH, pH 7.4 and 25C
21.7
mutant enzyme R168E, with acetoacetyl-CoA and NADH, pH 7.4 and 25C
22
mutant enzyme K152A, with acetoacetyl-CoA and NADH, pH 7.4 and 25C
25.5
mutant enzyme K152A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25C
31.8
mutant enzyme Q126E/K169E, with acetoacetyl-CoA and NADH, pH 7.4 and 25C
38.29
with ethyl 4-chloroacetoacetate as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0
42.6
-
mutant R187E/R230E, acyl-carrier protein-independent spectroscopic assay
45.6
-
mutant R187A/R230A, acyl-carrier protein-independent spectroscopic assay
54.2
-
mutant R230E, acyl-carrier protein-independent spectroscopic assay
54.5
-
mutant R187E, acyl-carrier protein-independent spectroscopic assay
55.3
-
mutant R230A, acyl-carrier protein-independent spectroscopic assay
57.9
-
mutant R187A, acyl-carrier protein-independent spectroscopic assay
70.6
-
wild-type, acyl-carrier protein-dependent spectroscopic assay
74.6
mutant enzyme D42A, with 9,10-phenanthrene quinone and NAD+, pH 7.4 and 25C
280
-
acetoacetyl-N-acetylcysteamine
450
-
acetoacetyl-CoA
530
-
acetoacteyl-ACP
3839
-
recombinant protein, cosubstrate NADH, pH 7.0, phosphate buffer
4930
-
purified enzyme
9401
-
purified enzyme, protein determination by amino acid analysis
83140
-
cosubstrate NADPH, pH 8.35, carbonate buffer
136000
-
purified enzyme, protein determination by calculation from absorption at 280 nm
180400
-
recombinant protein, cosubstrate NADPH, pH 7.0, phosphate buffer
additional information
the intrinsic NADPH dependent 3-ketoacyl-CoA reductase activity in the transformant Pseudomonas putida with overexpressed fabG gene increased about 1.6-fold from 0.87 to 1.39 units/mg protein, indicating successful overexpression of the fabGJ genes in Pseudomonas putida KCTC1639.