1.1.1.100: 3-oxoacyl-[acyl-carrier-protein] reductase

This is an abbreviated version, for detailed information about 3-oxoacyl-[acyl-carrier-protein] reductase, go to the full flat file.

Reaction

a (3R)-3-hydroxyacyl-[acyl-carrier protein]
+
NADP+
=
a 3-oxoacyl-[acyl-carrier protein]
+
NADPH
+
H+

Synonyms

3-ketoacyl acyl carrier protein reductase, 3-ketoacyl-(acyl-carrier-protein) reductase, 3-ketoacyl-ACP reductase, 3-ketoacyl-ACP(CoA) reductase, 3-ketoacyl-acyl carrier protein reductase, 3-ketoacyl-[acyl-carrier-protein] reductase, 3-oxoacyl-(acyl carrier protein) reductase, 3-oxoacyl-ACP reductase, 3-oxoacyl-AcpM reductase, 3-oxoacyl-thioester reductase, 3-oxoacyl-[ACP]reductase, ACP reductase, beta-ketoacyl acyl carrier protein (ACP) reductase, beta-ketoacyl acyl carrier protein reductase, beta-ketoacyl reductase, beta-ketoacyl thioester reductase, beta-ketoacyl-ACP reductase, beta-ketoacyl-acyl carrier protein reductase, beta-ketoacyl-[acyl carrier protein] reductase, beta-ketoacyl-[acyl-carrier protein] (ACP) reductase, BKR, FabG, FabG1, fabG4, KACPR, KCR1, KCR2, MabA, NADPH-specific 3-oxoacyl-[acylcarrier protein]reductase, OAR, Oar1p, PHA-specific acetoacetyl-CoA reductase, PhaB, polyhydroxyalkanoate-specific acetoacetyl coenzyme A reductase, reductase, 3-oxoacyl-[acyl carrier protein], RhlG

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.100 3-oxoacyl-[acyl-carrier-protein] reductase

Purification

Purification on EC 1.1.1.100 - 3-oxoacyl-[acyl-carrier-protein] reductase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by gel filtration and ion exchange chromatography, to homogeneity
by nickel affinity chromatography
-
by nickel chelation affinity chromatography
by nickel-affinity chromatography
-
by sonication, centrifugation and on Ni-NTA column
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) solubilized from inclusion bodies and refolded is further purified by nickel affinity chromatography
-
recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography
recombinant His10-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant protein
-
recombinant protein, modified purification protocol
-
using Ni-NTA chromatography
wild-type and mutants purified to homogeneity using an Ni2+-nitrilotriacetic acid affinity column
-