1.1.1.100: 3-oxoacyl-[acyl-carrier-protein] reductase

This is an abbreviated version, for detailed information about 3-oxoacyl-[acyl-carrier-protein] reductase, go to the full flat file.

Reaction

a (3R)-3-hydroxyacyl-[acyl-carrier protein]
+
NADP+
=
a 3-oxoacyl-[acyl-carrier protein]
+
NADPH
+
H+

Synonyms

3-ketoacyl acyl carrier protein reductase, 3-ketoacyl-(acyl-carrier-protein) reductase, 3-ketoacyl-ACP reductase, 3-ketoacyl-ACP(CoA) reductase, 3-ketoacyl-acyl carrier protein reductase, 3-ketoacyl-[acyl-carrier-protein] reductase, 3-oxoacyl-(acyl carrier protein) reductase, 3-oxoacyl-ACP reductase, 3-oxoacyl-AcpM reductase, 3-oxoacyl-thioester reductase, 3-oxoacyl-[ACP]reductase, ACP reductase, beta-ketoacyl acyl carrier protein (ACP) reductase, beta-ketoacyl acyl carrier protein reductase, beta-ketoacyl reductase, beta-ketoacyl thioester reductase, beta-ketoacyl-ACP reductase, beta-ketoacyl-acyl carrier protein reductase, beta-ketoacyl-[acyl carrier protein] reductase, beta-ketoacyl-[acyl-carrier protein] (ACP) reductase, BKR, FabG, FabG1, fabG4, KACPR, KCR1, KCR2, MabA, NADPH-specific 3-oxoacyl-[acylcarrier protein]reductase, OAR, Oar1p, PHA-specific acetoacetyl-CoA reductase, PhaB, polyhydroxyalkanoate-specific acetoacetyl coenzyme A reductase, reductase, 3-oxoacyl-[acyl carrier protein], RhlG

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.100 3-oxoacyl-[acyl-carrier-protein] reductase

Cloned

Cloned on EC 1.1.1.100 - 3-oxoacyl-[acyl-carrier-protein] reductase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
coexpression in Escherichia coli LS5218 with fabH mutant F87T and polyhydroxyalkanoate synthase genes
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complementation of Escherichia coli fabG mutant with Lactobacillus lactis fabG genes shows that fabG1 encodes a functional beta-ketoacyl ACP reductase and fabG2 encodes an enzyme that has activity with the model substrate beta-ketobutyryl-CoA, but lacks the ability to support fatty acid synthesis both in vivo and in vitro
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expressed in Escherichia coli
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expressed in Escherichia coli as a His-tagged fusion protein
expression in Escherichia coli
expression in Saccharomyces cerevisae mutant cells lacking the homologous mitochondrial FASII enzyme 3-oxoacyl-ACP reductase Oar1p
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expression in Streptomyces cerevisiae cells lacking 3-oxoacyl-ACP reductase; FabG1 expressed from vector pPLM189 as mitochondrial protein in the Saccharomyces cerevisiae oar1DELTA mutant strain. FabG1 can restore respiratory growth of Saccharomyces cerevisiae oar1DELTA cells and renews lipoic acid production; FabG4 expressed from vector pPLM192 as mitochondrial protein in the Saccharomyces cerevisiae oar1DELTA mutant strain. FabG4 can restore respiratory growth of Saccharomyces cerevisiae oar1DELTA cells and renews lipoic acid production
expression of protein without transit peptide in Escherichia coli
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gene fabG, DNA and amino acid sequence determination and analysis, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
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gene fabG, DNA and amino acid sequence determination and analysis, expression of His10-tagged enzyme in Escherichia coli
gene fabG, expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
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gene fabG, overexpression of His-tagged enzyme in strain BL21(DE3)
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gene fabG, overexpression of His-tagged enzyme in strain BL21(DE3), recombinant expression in Escherichia coli strain JM109 leads to enhanced production of polyhydroxyalkanoate, PHA, copolymer from glucose, PHA composition, overview
gene rhlG, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) or, as selenomethionine-labeled variant in Escherichia coli strain B834 in minimal medium
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heterologous coexpression of the polyhydroxyalkanoate synthase genes phaEC together with fabG1 in Haloferax volcanii
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heterologous coexpression of the polyhydroxyalkanoate synthase genes phaEC together with Haloarcula hispanica fabG1 reconstructs the polyhydroxyalkanoate biosynthetic pathway
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His-tagged FabG expressed in Escherichia coli strain BL21 (DE3)
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into pET-28a vector and expressed in Escherichia coli BL21 (DE3) codon plus
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NH2-terminally His-tagged FabG expressed in Escherichia coli strain BL21(DE3)
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overexpressed in Escherichia coli BL21 (DE3)
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overexpression in Escherichia coli strain BL21 (DE3)
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overexpression of fabG gene in Escherichia coli strain BL21 (DE3)
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overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) in inclusion bodies
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PCR product recombined with pDONR221 and insert from this vector transferred in the LR reaction to the expression vector pET15g which adds a histidine tag and a 3C protease cleavage site, expressed in Escherichia coli B834(DE3) cells
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pET15b-fabG vector construct transformed into the Escherichia coli BL21 DE3 strain
Recombinant Escherichia coli (strain DH5alpha) co-expresses the fabG gene. The fabG gene is PCR-cloned using primers derived from the up and down-stream regions of the Pseudomonas putida KT2440 genome.
selection of an Escherichia coli, BL21(DE3)NH host that stably expresses mutant forms of 3-ketoacyl-ACP(CoA) reductase
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transformation of the fabG gene encoding 3-ketoacyl-[acyl-carrier-protein] reductase in fusion with a His6-MBP-tag (ligated into vector pETM-41) into Escherichia coli XL-10 Gold. After isolation from XL-10 cells and DNA sequencing, transformed into Escherichia coli Tuner (DE3) cells
transformation with 3-oxoacyl-[acyl-carrier-protein] reductase in antisense orientation, driven by either the cauliflower mosaic virus 35S promoter or a seed-specific acyl carrier protein promoter
wild-type and mutants cloned into the pET-28a(+) vector and expressed in Escherichia coli BL21 (DE3) codon plus
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YFP-KCR1 and CFP-KCR2 fusion constructs transiently expressed in tobacco under the control of the 35S promoter or in Arabidopsis. KCR1 and KCR2 expressed in the yeast ybr159DELTA mutant
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