1.1.1.10: L-xylulose reductase

This is an abbreviated version, for detailed information about L-xylulose reductase, go to the full flat file.

Reaction

xylitol
+
NADP+
=
L-xylulose
+
NADPH
+
H+

Synonyms

DCXR, dicarbonyl/L-xylulose reductase, L-xylulose reductase, LXR3, More, NAD(P)H-dependent xylose reductase, NADP(+)-dependent xylitol dehydrogenase, P31h, P34H, reductase, L-xylulose, XDH, XR, xylitol dehydrogenase, xylose reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.10 L-xylulose reductase

Crystallization

Crystallization on EC 1.1.1.10 - L-xylulose reductase

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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
18 mg/ml purified enzyme complexed with NADPH, in 10 mM Tris-HCl, pH 7.5, 2 mM 2-mercaptoethanol, 20% glycerol, replacement buffer is 10 mM Tris-HCl, pH 7.5, 2 mM 2-mercaptoethanol, mixed with 12.9 mM NADPH, in a molar ratio of enzyme and cofactor of 1:8, equal volume of 0.003 ml of enzyme complex mixture and well solution, containing 15% PEG 8000, 50 mM potassium phosphate, and 0.1 M MES, pH 6.5, 1 week, X-ray diffraction structure determination and analysis, molecular replacement method, 1.96 A resolution, molecular modeling
-
crystal structure analysis and modelling
-
structure of the biological tetramer of human L-xylulose reductase in complex with NADP+ and the competitive inhibitor 4-methyl-[1,2,3]-thiadiazole-5-carboxylic acid benzyloxyamide, vapor diffusion method
-
vapour diffusion method, using 15% PEG 8000, 0.05 M potassium phosphate and 0.1 M MES buffer, pH 6.5
-