1.1.1.1: alcohol dehydrogenase

This is an abbreviated version, for detailed information about alcohol dehydrogenase, go to the full flat file.

Reaction

a primary alcohol
+
NAD+
=
an aldehyde
+
NADH
+
H+

Synonyms

(R)-specific alcohol dehydrogenase, 40 kDa allergen, Aadh1, ADH, ADH 1, ADH class III, ADH I, ADH II, ADH-10, ADH-A, ADH-A2, ADH-B2, ADH-C2, ADH-HT, ADH-I, ADH1, ADH1B, ADH1C, ADH1C*1, ADH1C*2, Adh1p, ADH2, ADH3, ADH4, ADH5, ADH6Hp, ADH8, AdhA, AdhB, AdhC, AdhD, AdhE, ADS1, AFPDH, alcohol dehydrogenase, alcohol dehydrogenase (NAD), alcohol dehydrogenase 1, alcohol dehydrogenase 10, alcohol dehydrogenase 2, alcohol dehydrogenase 3, alcohol dehydrogenase 5, alcohol dehydrogenase I, alcohol dehydrogenase II, Alcohol dehydrogenase-B2, alcohol dependent dehydrogenase, alcohol-aldehyde/ketone oxidoreductase, NAD+-dependent, alcohol:NAD+ oxidoreductase, aldehyde dehydrogenase, aldehyde reductase, aldehyde/alcohol dehydrogenase, ALDH, aliphatic alcohol dehydrogenase, APE2239, APE_2239.1, ARAD1B16786p, bifunctional alcohol/aldehyde dehydrogenase, CHY1186, class I ADH, class I ALDH, class II ADH, class III ADH, class III alcohol dehydrogenase, class IV ADH, Cm-ADH2, Cthe_0423, DADH, dehydrogenase, alcohol, ethanol dehydrogenase, FALDH, FDH, Gastric alcohol dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, GSH-FDH, HLAD, HpADH3, HtADH, HvADH1, HVO_2428, iron-containing alcohol dehydrogenase, KlADH4, KlDH3, KmADH3, KmADH4, long-chain alkyl alcohol dehydrogenase, LSADH, medium chain alcohol dehydrogenase, medium-chain NAD+-dependent ADH, medium-chain secondary alcohol dehydrogenase, NAD(H)-dependent alcohol dehydrogenase, NAD+-ADH, NAD+-dependent (S)-stereospecific alcohol dehydrogenase, NAD+-dependent alcohol dehydrogenase, NAD-dependent alcohol dehydrogenase, NAD-dependent medium-chain ADH, NAD-specific aromatic alcohol dehydrogenase, NADH-alcohol dehydrogenase, NADH-aldehyde dehydrogenase, NADH-dependent alcohol dehydrogenase, Octanol dehydrogenase, PF0991 protein, PF1960, primary alcohol dehydrogenase, Retinol dehydrogenase, SaADH, SaADH2, Saci_1232, SADH, SCAD, sec-ADH A, short-chain ADH, short-chain NAD(H)-dependent dehydrogenase/reductase, SSADH, SsADH-10, SSO2536, ST0053, Ta1316 ADH, TaDH, TBADH, Teth39_0206, Teth39_0218, Teth514_0627, TK0845, Tsac_0416, Y-ADH, YADH, YADH-1, yeast alcohol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.1 alcohol dehydrogenase

pH Stability

pH Stability on EC 1.1.1.1 - alcohol dehydrogenase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.3
30 min, 50°C, 50% loss of activity
718627
4 - 8
4°C, 48 h, stable
684556
4
-
30°C, 1 h, 30% loss of activity
686626
4 - 10
30 min, 50°C, no loss of activity
718627
5 - 9.5
-
25°C, 1 h, stable
667255
5
-
30°C, 1 h, 20% loss of activity
686626
5.2 - 6.3
-
purified recombinant enzyme expressed from Saccharomyces cerevisiae, at least 80% of the initial activity is retained after 4 min, 30°C
722888
5.5 - 6.3
-
purified recombinant enzyme expressed from Hansenula polymorpha, at least 80% of the initial activity is retained after 4 min, 30°C
722888
5.8 - 6.6
-
purified recombinant enzyme expressed from Arxula adeninivorans, at least 80% of the initial activity is retained after 4 min, 30°C
722888
6 - 11
-
30°C, 1 h, less than 10% loss of activity
686626
6 - 8
-
at pH 6.0 and 8.0, the activity of free ADH decreases dramatically during the incubation, and 90 min later most of the activity is lost, the immobilized form retains 81% of activity at pH 8.0
695705
6 - 9
-
isoenzyme 2 and isoenzyme 3
285630
6.5 - 7
-
most stable at
285629
6.5 - 9
-
native Zn-ADH enzyme
655741
6.5
-
below, Cu-ADH and Co-ADH
655741
7 - 9
with 100 mM diphosphate buffer at pH 9.0, the enzyme only exhibits 49% of activity, a 50% decrease is obtained using 50 mM bis-Tris propane buffer at pH 7.0-7.5 and 9.0, whereas at pH 8 and 8.5 only a 10% reduction is found
699249
7 - 10.6
-
stable
285567
7.5
-
4°C, stable for 2 days
285623
8 - 9
purified recombinant His-tagged enzyme, 10 min, high stability within this range
722337
11
-
rapid loss of activity
739588