1.1.1.1: alcohol dehydrogenase

This is an abbreviated version, for detailed information about alcohol dehydrogenase, go to the full flat file.

Reaction

a primary alcohol
+
NAD+
=
an aldehyde
+
NADH
+
H+

Synonyms

(R)-specific alcohol dehydrogenase, 40 kDa allergen, Aadh1, ADH, ADH 1, ADH class III, ADH I, ADH II, ADH-10, ADH-A, ADH-A2, ADH-B2, ADH-C2, ADH-HT, ADH-I, ADH1, ADH1B, ADH1C, ADH1C*1, ADH1C*2, Adh1p, ADH2, ADH3, ADH4, ADH5, ADH6Hp, ADH8, AdhA, AdhB, AdhC, AdhD, AdhE, ADS1, AFPDH, alcohol dehydrogenase, alcohol dehydrogenase (NAD), alcohol dehydrogenase 1, alcohol dehydrogenase 10, alcohol dehydrogenase 2, alcohol dehydrogenase 3, alcohol dehydrogenase 5, alcohol dehydrogenase I, alcohol dehydrogenase II, Alcohol dehydrogenase-B2, alcohol dependent dehydrogenase, alcohol-aldehyde/ketone oxidoreductase, NAD+-dependent, alcohol:NAD+ oxidoreductase, aldehyde dehydrogenase, aldehyde reductase, aldehyde/alcohol dehydrogenase, ALDH, aliphatic alcohol dehydrogenase, APE2239, APE_2239.1, ARAD1B16786p, bifunctional alcohol/aldehyde dehydrogenase, CHY1186, class I ADH, class I ALDH, class II ADH, class III ADH, class III alcohol dehydrogenase, class IV ADH, Cm-ADH2, Cthe_0423, DADH, dehydrogenase, alcohol, ethanol dehydrogenase, FALDH, FDH, Gastric alcohol dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, GSH-FDH, HLAD, HpADH3, HtADH, HvADH1, HVO_2428, iron-containing alcohol dehydrogenase, KlADH4, KlDH3, KmADH3, KmADH4, long-chain alkyl alcohol dehydrogenase, LSADH, medium chain alcohol dehydrogenase, medium-chain NAD+-dependent ADH, medium-chain secondary alcohol dehydrogenase, NAD(H)-dependent alcohol dehydrogenase, NAD+-ADH, NAD+-dependent (S)-stereospecific alcohol dehydrogenase, NAD+-dependent alcohol dehydrogenase, NAD-dependent alcohol dehydrogenase, NAD-dependent medium-chain ADH, NAD-specific aromatic alcohol dehydrogenase, NADH-alcohol dehydrogenase, NADH-aldehyde dehydrogenase, NADH-dependent alcohol dehydrogenase, Octanol dehydrogenase, PF0991 protein, PF1960, primary alcohol dehydrogenase, Retinol dehydrogenase, SaADH, SaADH2, Saci_1232, SADH, SCAD, sec-ADH A, short-chain ADH, short-chain NAD(H)-dependent dehydrogenase/reductase, SSADH, SsADH-10, SSO2536, ST0053, Ta1316 ADH, TaDH, TBADH, Teth39_0206, Teth39_0218, Teth514_0627, TK0845, Tsac_0416, Y-ADH, YADH, YADH-1, yeast alcohol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.1 alcohol dehydrogenase

Specific Activity

Specific Activity on EC 1.1.1.1 - alcohol dehydrogenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.02
-
mutant recombinant enzyme E97C, from crude extract, at 65C
0.05
-
wild-type, cosubstrate NADPH, pH 7.0, 55C
0.07
-
gene AdhE deletion mutant, cosubstrate NADPH, pH 7.0, 55C
0.092
-
partially purified enzyme
0.1
-
isoenzyme 4
0.12
-
isoenzyme 1
0.18
after 5.67fold purification, using benzylalcohol as substrate, at 65C
0.22
crude extract, at pH 10.0
0.25
gene AdhE deletion mutant, cosubstrate NADPH, pH 7.0, 55C
0.3
-
substrates 1,3-propanediol and NAD+, pH 9.0, 60C, recombinant enzyme, with NiCl2 addition
0.5
-
purified mutant enzyme W95L, using benzyl alcohol as substrate, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65C
0.64
-
wild type enzyme, crude extract, at 65C
0.65
-
-
0.68
wild-type, cosubstrate NADPH, pH 7.0, 55C
0.75
purified enzyme, using benzylalcohol as substrate, at 80C
0.78
-
crude extract, at pH 10.0
1.1
-
substrates ethanol and NAD+, pH 9.0, 60C, recombinant enzyme
1.28
-
purified enzyme
1.36
-
isoenzyme A2
1.47
-
-
1.52
wild-type, cosubstrate NADH, pH 7.0, 55C
1.7
-
cofactor NAD+, pH 7.0, 30C
2 - 2.5
-
substrate diacetyl-acetoin, maximal specific activity detected in reduction reaction, 70C, pH 6.1
2.1
-
isoenzyme AA-ADH
2.6
in Tris-HCl buffer (pH 7.6), 5 mM dithiothreitol, 0.2 mM NADH, and 10 mM acetaldehyde at 65C
2.88
after 12.9fold purification, at pH 10.0
3.2
-
reduction of 3-oxo-5beta-androstan-17beta-ol
4.03
-
recombinant wild type enzyme, after 6fold purification, at 65C
4.1
-
wild type enzyme, at 65C
4.5
-
wild type recombinant enzyme, after 450fold purification, at 65C
4.75
-
purified recombinant enzyme, pH 6.5, 50C
5.18
-
pH 6.7, 30C, mutant enzyme S109P/L116S/Y294C
5.2
-
mutant recombinant enzyme E97C, after 260fold purification, at 65C
7.68
-
wild-type, cosubstrate NADH, pH 7.0, 55C
8.08
-
isoenzyme B2
10.1
-
after 12.9fold purification, at pH 10.0
15
crude enzyme, after heat treatment at 75C for 20 min
17.6
crude extract Ta1316 ADH with ethanol as a substrate, at pH 5.0 and 75C
18.6
-
purified mutant enzyme W95L/N249Y, using benzyl alcohol as substrate, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65C
20.6
after 1.4fold purifictaion
26.1
-
mutant enzyme N249Y, at 65C
27.6
-
purified native enzyme, pH 6.5, 30C
32.5
-
ADH-1
49.3
-
-
64
-
isoenzyme C2
65
-
mutant enzyme Adh1-1S1108
70
-
wild-type enzyme Adh1-1S
79.6
substrate butan-1-ol, pH 6.5, 60C
93
-
enzyme form ADHI
108
-
substrate butan-2,3-diol, maximal specific activity detected in oxidation reaction, 70C, pH 8.8
196.3
Sporotrichum pulverulentum
-
-
361
-
purified ADH II
447
substrate ethanol, pH 8.0, 60C
470
-
enzyme form ADHII
597
-
purified ADH I
628.7
purified Ta1316 ADH with ethanol as a substrate, at pH 5.0 and 75C
additional information