1.1.1.1: alcohol dehydrogenase

This is an abbreviated version, for detailed information about alcohol dehydrogenase, go to the full flat file.

Reaction

a primary alcohol
+
NAD+
=
an aldehyde
+
NADH
+
H+

Synonyms

(R)-specific alcohol dehydrogenase, 40 kDa allergen, Aadh1, acetaldehyde-alcohol dehydrogenase, ADH, ADH 1, ADH class III, ADH I, ADH II, ADH-10, ADH-A, ADH-A2, ADH-B2, ADH-C2, ADH-HT, ADH-I, ADH1, ADH1B, ADH1C, ADH1C*1, ADH1C*2, Adh1p, ADH2, ADH3, ADH4, ADH5, ADH6Hp, ADH8, AdhA, AdhB, AdhC, AdhD, AdhE, ADHES77, ADS1, AFPDH, alcohol dehydrogenase, alcohol dehydrogenase (NAD), alcohol dehydrogenase 1, alcohol dehydrogenase 10, alcohol dehydrogenase 2, alcohol dehydrogenase 3, alcohol dehydrogenase 5, alcohol dehydrogenase GroES domain protein, alcohol dehydrogenase I, alcohol dehydrogenase II, Alcohol dehydrogenase-B2, alcohol dependent dehydrogenase, alcohol-aldehyde/ketone oxidoreductase, NAD+-dependent, alcohol:NAD+ oxidoreductase, aldehyde dehydrogenase, aldehyde reductase, aldehyde/alcohol dehydrogenase, ALDH, aliphatic alcohol dehydrogenase, alpha-ketoaldehyde dehydrogenase, APE2239, APE_2239.1, ARAD1B16786p, bifunctional acetaldehyde-alcohol dehydrogenase, bifunctional alcohol/aldehyde dehydrogenase, CHY1186, class I ADH, class I ALDH, class II ADH, class III ADH, class III alcohol dehydrogenase, class IV ADH, Cm-ADH2, Cthe_0423, DADH, dehydrogenase, alcohol, ethanol dehydrogenase, FALDH, FDH, Gastric alcohol dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, GSH-FDH, HLAD, HpADH3, HtADH, HvADH1, HVO_2428, iron-containing alcohol dehydrogenase, KlADH4, KlDH3, KmADH3, KmADH4, long-chain alkyl alcohol dehydrogenase, LSADH, medium chain alcohol dehydrogenase, medium-chain NAD+-dependent ADH, medium-chain secondary alcohol dehydrogenase, MGD, NAD(H)-dependent alcohol dehydrogenase, NAD+-ADH, NAD+-dependent (S)-stereospecific alcohol dehydrogenase, NAD+-dependent alcohol dehydrogenase, NAD+-linked alcohol dehydrogenase 1, NAD+-linked methylglyoxal dehydrogenase, NAD-dependent alcohol dehydrogenase, NAD-dependent medium-chain ADH, NAD-specific aromatic alcohol dehydrogenase, NADH-alcohol dehydrogenase, NADH-aldehyde dehydrogenase, NADH-dependent alcohol dehydrogenase, Octanol dehydrogenase, Pcal_1311, PF0991 protein, PF1960, primary alcohol dehydrogenase, Retinol dehydrogenase, SaADH, SaADH2, Saci_1232, SADH, SCAD, sec-ADH A, short-chain ADH, short-chain NAD(H)-dependent dehydrogenase/reductase, SSADH, SsADH-10, SSO2536, ST0053, Ta1316 ADH, TaDH, TBADH, Teth39_0206, Teth39_0218, Teth514_0627, TK0845, Tsac_0416, Y-ADH, YADH, YADH-1, yeast alcohol dehydrogenase, YLL056C

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.1 alcohol dehydrogenase

Specific Activity

Specific Activity on EC 1.1.1.1 - alcohol dehydrogenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.02
-
mutant recombinant enzyme E97C, from crude extract, at 65C
0.05
-
wild-type, cosubstrate NADPH, pH 7.0, 55C
0.07
-
gene AdhE deletion mutant, cosubstrate NADPH, pH 7.0, 55C
0.092
-
partially purified enzyme
0.1
-
isoenzyme 4
0.12
-
isoenzyme 1
0.18
after 5.67fold purification, using benzylalcohol as substrate, at 65C
0.22
crude extract, at pH 10.0
0.25
I3VSF1
gene AdhE deletion mutant, cosubstrate NADPH, pH 7.0, 55C
0.3
-
substrates 1,3-propanediol and NAD+, pH 9.0, 60C, recombinant enzyme, with NiCl2 addition
0.44
-
substrate formaldehyde, pH 7.0, 30C
0.47
-
substrate furfural, pH 7.0, 30C
0.5
-
purified mutant enzyme W95L, using benzyl alcohol as substrate, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65C
0.64
-
wild type enzyme, crude extract, at 65C
0.65
-
-
0.68
I3VSF1
wild-type, cosubstrate NADPH, pH 7.0, 55C
0.75
purified enzyme, using benzylalcohol as substrate, at 80C
0.78
-
crude extract, at pH 10.0
1.1
-
substrates ethanol and NAD+, pH 9.0, 60C, recombinant enzyme
1.28
-
purified enzyme
1.36
-
isoenzyme A2
1.47
-
-
1.52
I3VSF1
wild-type, cosubstrate NADH, pH 7.0, 55C
2 - 2.5
-
substrate diacetyl-acetoin, maximal specific activity detected in reduction reaction, 70C, pH 6.1
2.1
-
isoenzyme AA-ADH
2.6
in Tris-HCl buffer (pH 7.6), 5 mM dithiothreitol, 0.2 mM NADH, and 10 mM acetaldehyde at 65C
2.88
after 12.9fold purification, at pH 10.0
3.2
-
reduction of 3-oxo-5beta-androstan-17beta-ol
4.03
-
recombinant wild type enzyme, after 6fold purification, at 65C
4.1
-
wild type enzyme, at 65C
4.5
-
wild type recombinant enzyme, after 450fold purification, at 65C
4.75
-
purified recombinant enzyme, pH 6.5, 50C
5.18
-
pH 6.7, 30C, mutant enzyme S109P/L116S/Y294C
5.2
-
mutant recombinant enzyme E97C, after 260fold purification, at 65C
6.5
-
substrate glycolaldehyde, pH 7.0, 30C
7.68
-
wild-type, cosubstrate NADH, pH 7.0, 55C
8.08
-
isoenzyme B2
10.1
-
after 12.9fold purification, at pH 10.0
15
crude enzyme, after heat treatment at 75C for 20 min
17.6
crude extract Ta1316 ADH with ethanol as a substrate, at pH 5.0 and 75C
18.6
-
purified mutant enzyme W95L/N249Y, using benzyl alcohol as substrate, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65C
20.6
after 1.4fold purifictaion
26.1
-
mutant enzyme N249Y, at 65C
27.6
-
purified native enzyme, pH 6.5, 30C
32.5
-
ADH-1
64
-
isoenzyme C2
65
-
mutant enzyme Adh1-1S1108
70
-
wild-type enzyme Adh1-1S
79.6
substrate butan-1-ol, pH 6.5, 60C
93
-
enzyme form ADHI
108
-
substrate butan-2,3-diol, maximal specific activity detected in oxidation reaction, 70C, pH 8.8
196.3
Sporotrichum pulverulentum
-
-
361
-
purified ADH II
447
substrate ethanol, pH 8.0, 60C
470
-
enzyme form ADHII
597
-
purified ADH I
628.7
purified Ta1316 ADH with ethanol as a substrate, at pH 5.0 and 75C
additional information