1.1.1.1: alcohol dehydrogenase

This is an abbreviated version, for detailed information about alcohol dehydrogenase, go to the full flat file.

Reaction

a primary alcohol
+
NAD+
=
an aldehyde
+
NADH
+
H+

Synonyms

(R)-specific alcohol dehydrogenase, 40 kDa allergen, Aadh1, ADH, ADH 1, ADH class III, ADH I, ADH II, ADH-10, ADH-A, ADH-A2, ADH-B2, ADH-C2, ADH-HT, ADH-I, ADH1, ADH1B, ADH1C, ADH1C*1, ADH1C*2, Adh1p, ADH2, ADH3, ADH4, ADH5, ADH6Hp, ADH8, AdhA, AdhB, AdhC, AdhD, AdhE, ADS1, AFPDH, alcohol dehydrogenase, alcohol dehydrogenase (NAD), alcohol dehydrogenase 1, alcohol dehydrogenase 10, alcohol dehydrogenase 2, alcohol dehydrogenase 3, alcohol dehydrogenase 5, alcohol dehydrogenase I, alcohol dehydrogenase II, Alcohol dehydrogenase-B2, alcohol dependent dehydrogenase, alcohol-aldehyde/ketone oxidoreductase, NAD+-dependent, alcohol:NAD+ oxidoreductase, aldehyde dehydrogenase, aldehyde reductase, aldehyde/alcohol dehydrogenase, ALDH, aliphatic alcohol dehydrogenase, APE2239, APE_2239.1, ARAD1B16786p, bifunctional alcohol/aldehyde dehydrogenase, CHY1186, class I ADH, class I ALDH, class II ADH, class III ADH, class III alcohol dehydrogenase, class IV ADH, Cm-ADH2, Cthe_0423, DADH, dehydrogenase, alcohol, ethanol dehydrogenase, FALDH, FDH, Gastric alcohol dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, GSH-FDH, HLAD, HpADH3, HtADH, HvADH1, HVO_2428, iron-containing alcohol dehydrogenase, KlADH4, KlDH3, KmADH3, KmADH4, long-chain alkyl alcohol dehydrogenase, LSADH, medium chain alcohol dehydrogenase, medium-chain NAD+-dependent ADH, medium-chain secondary alcohol dehydrogenase, NAD(H)-dependent alcohol dehydrogenase, NAD+-ADH, NAD+-dependent (S)-stereospecific alcohol dehydrogenase, NAD+-dependent alcohol dehydrogenase, NAD-dependent alcohol dehydrogenase, NAD-dependent medium-chain ADH, NAD-specific aromatic alcohol dehydrogenase, NADH-alcohol dehydrogenase, NADH-aldehyde dehydrogenase, NADH-dependent alcohol dehydrogenase, Octanol dehydrogenase, PF0991 protein, PF1960, primary alcohol dehydrogenase, Retinol dehydrogenase, SaADH, SaADH2, Saci_1232, SADH, SCAD, sec-ADH A, short-chain ADH, short-chain NAD(H)-dependent dehydrogenase/reductase, SSADH, SsADH-10, SSO2536, ST0053, Ta1316 ADH, TaDH, TBADH, Teth39_0206, Teth39_0218, Teth514_0627, TK0845, Tsac_0416, Y-ADH, YADH, YADH-1, yeast alcohol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.1 alcohol dehydrogenase

Purification

Purification on EC 1.1.1.1 - alcohol dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
128fold to homogeneity by adenosine 5'-monophosphate affinity and Mono-Q ion exchange chromatography
-
2 isozymes from cytosol by DEAE ion exchange, hydroxyl apatite, and gel filtration chromatography, ADH I 35fold, ADH II 21fold
-
3 isoenzymes
-
ADH-1, ADH-2 and ADH-3
-
ADH-2 and ADH-3
-
ADH-MII
-
ADHE cannot be solubilized from membrane with detergents such as 1% Triton X-100 or 1% sulfobetaine 3-12. The enzyme is easily dissociated from membrane by high-salt buffers containing either 1.0 M NaCl or (NH4)2SO4 without detergents
-
alleloenzyme Adh71k
-
alleloenzymes Adhf and Adhus
-
anodic enzyme form
-
Blue A column chromatography
-
class I isoenzyme
-
class I isoenzymes
-
class II isoenzyme: pi-ADH
-
class III isoenzyme chi-ADH
-
DEAE-Sepharose CL-4B column chromatograhy and octyl-Sepharose column chromatography
DEAE-Sepharose column chromatography
DEAE-Sepharose Fast Flow column chromatography and G75 gel filtration
-
DEAE-Sepharose Fast Flow column chromatography, Matrex Gel Red A column chromatography, and Blue A column chromatography
-
HiLoad Superdex 200 gel filtration
HIS-Select High Flow cartridge chromatography
HiTrap heparin-Sepharose column chromatography, DEAE-Sepharose fast-flow column chromatography, and HiLoad Superdex S-75 gel filtration
-
isoenzyme 1, 2, 3, and 4
-
isoenzyme beta3,beta3
-
liver isoenzyme A2 and B2 and stomach isoenzyme C2
-
mutant enzyme S109P/L116S/Y294
-
native (R)-specific alcohol dehydrogenase 37fold from strain IFO10003, by ammonium sulfate fractionation, and anion exchange and hydrophobic interaction chromatography to homogeneity
-
native enzyme from liver by anion exchange and AMP affinity chromatography, and a second different step of anion exchange chromatography
partial
partial by isopycnic sucrose gradient centrifugation
-
purified in one step by immobilized metal-affinity chromatography
recombinant ADH2 alloenzymes from Escherichia coli by DEAE and AMP or blue Sepharose chromatography, and ultrafiltration
-
recombinant enzyme
-
recombinant enzyme 1.5fold from Escherichia coli strain BL21 (DE3)
-
recombinant enzyme from Escherichia coli by DEAE ion exchange, 5'-AMP-resin affinity, and Mono Q ion exchange chromatography
-
recombinant His-tagged enzyme by nickel affinity chromatography and gel filtration
-
recombinant His6-tagged enzyme from Echerichia coli strain BL21 (DE3) by nickel affinity chromatography
recombinant isozymes from Escherichia coli strain BL21
reombinant fusion enzyme by glutathione affinity chromatography, cleavage of GST fusion tag by thrombin, further purification of the active enzyme
-
Sephacryl S-100 gel filtration
soluble recombinant His6-tagged BmADH protein from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
SS-isoenzyme
-
Talon Co2+-affinity column chromatography
Toyopearl Butyl 650 S column chromatography, Q Sepharose fast flow column chromatography, Superose 12 prep-grade gel filtration, and TosoHaas G 2000 SWXL gel filtration
-
ultracentrifugation and Sepabeads EB-QA405 chromatography
using reactive Green 19 covalently immobilized ontomagnetic poly(2-hydroxyethyl methacrylate) nanostructures. Maximum alcohol dehydrogenase adsorption capacity is 176.09 mg/g polymer. Alcohol dehydrogenase molecules are desorbed by using 1.0 M NaCl with 98.4% recovery, purification is 45.63fold in a single step
-
wild-type enzyme Adh1-1S and mutant enzyme Adh1-1S1108
-